KMT2A/MLL Antibody

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Biological Strategies: Western Blot: KMT2A/MLL Antibody [NB600-248] - Samples: Nuclear extract (50 and 15 ug) from HeLa, 293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB ...read more
Immunoprecipitation: KMT2A/MLL Antibody [NB600-248] - Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MLL1 antibody NB600-248 used ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

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KMT2A/MLL Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 720 and 780 of human myeloid/lymphoid or mixed-lineage leukemia 1 using the number given in Swiss-Prot entry Q03164 (GeneID 4297).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KMT2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:250
  • Immunoprecipitation 2 - 10 ug/mg of lysate
  • Western Blot 1:500-1:5000
Application Notes
Use in IHC-P reported in scientific literature (PMID: 27396339).
Reviewed Applications
Read 1 Review rated 4
using
NB600-248 in the following applications:

Publications
Read Publications using
NB600-248 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31786140).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for KMT2A/MLL Antibody

  • ALL1
  • ALL-1MLL/GAS7
  • CXXC7TET1-MLL
  • CXXC-type zinc finger protein 7
  • EC 2.1.1.43
  • HRXFLJ11783
  • HTRX
  • HTRX1MLL-AF4 der(11) fusion protein
  • KMT2ACDK6/MLL fusion protein
  • Lysine N-methyltransferase 2A
  • MLL/GAS7 fusion protein
  • MLL/GMPS fusion protein
  • MLL1
  • MLL1A
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog)
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila)
  • Trithorax-like protein
  • TRX1histone-lysine N-methyltransferase MLL
  • Zinc finger protein HRX

Background

A partial tandem duplication within the MLL-gene (MLL-PTD) can be found in 8% of all patients with karyotypically normal acute myeloid leukemia (AML). MLL possibly acts as a transcriptional regulatory factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KMT2A/MLL Antibody (NB600-248)(29)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Chemotaxis, IHC-P, Immunoprecipitation, RIP.


Filter By Application
Chemotaxis
(1)
IHC-P
(1)
Immunoprecipitation
(1)
RIP
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NB600-248 Applications Species
Tianyu Zou, Kazuo Sugimoto, Yu Zhao, Baitao Li, Xiaomao Zhou, Cheng Peng Zhi‐zi‐chi decoction mitigates depression by enhancing lncRNA Six3os1 expression and promoting histone H3K4 methylation at the BDNF promoter Journal of Cellular and Molecular Medicine 2024-05-31 [PMID: 38818577]
Oelschl�ger L, Stahl P, Kaschani F et al. Taspase1 Facilitates Topoisomerase II?-Mediated DNA Double-Strand Breaks Driving Estrogen-Induced Transcription Cells 2023-01-18 [PMID: 36766705] (Immunoprecipitation) Immunoprecipitation
Zhu G, Luo H, Feng Y et al. HOXBLINC long non-coding RNA activation promotes leukemogenesis in NPM1-mutant acute myeloid leukemia Nature communications 2021-03-29 [PMID: 33782403] (RIP, Chemotaxis, Human) RIP, Chemotaxis Human
Luo H, Zhu G, Xu J et al. HOTTIP lncRNA Promotes Hematopoietic Stem Cell Self-Renewal Leading to AML-like Disease in Mice Cancer Cell 2019-11-27 [PMID: 31786140] (Mouse) Mouse
Barrett NA, Malouf C, Kapeni C et al. Mll-AF4 Confers Enhanced Self-Renewal and Lymphoid Potential during a Restricted Window in Development Cell Rep 2016-07-26 [PMID: 27396339] (IHC-P) IHC-P
Shore AN, Kabotyanski EB, Roarty K et al. Pregnancy-induced noncoding RNA (PINC) associates with polycomb repressive complex 2 and regulates mammary epithelial differentiation. PLoS Genet 2012-01-01 [PMID: 22911650]
Liu X, Qu X, Chen Y et al. Mesenchymal stem/stromal cells induce the generation of novel IL-10-dependent regulatory dendritic cells by SOCS3 activation. J Immunol 2012-08-01 [PMID: 22753940]
Akalin A, Garrett-Bakelman FE, Kormaksson M et al. Base-pair resolution DNA methylation sequencing reveals profoundly divergent epigenetic landscapes in acute myeloid leukemia. PLoS Genet 2012-01-01 [PMID: 22737091]
Zhang Y, Liu Z, Medrzycki M et al. Reduction of Hox gene expression by histone H1 depletion. PLoS One 2012-01-01 [PMID: 22701719]
Wang QF, Wu G, Mi S et al. MLL fusion proteins preferentially regulate a subset of wild-type MLL target genes in the leukemic genome. Blood 2011-06-01 [PMID: 21518926]
Show All 29 Publications.

Review for KMT2A/MLL Antibody (NB600-248) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB600-248:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot KMT2A/MLL NB600-248
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4
reviewed by:
Verified Customer
WB Human 08/27/2014
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Summary

ApplicationWestern Blot
Sample TestedCell lysate from Jurkat cells grown in RPMI medium
SpeciesHuman

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Recent Reviews

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Verified Customer
08/27/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol KMT2A
Entrez
Uniprot