![Western Blot: KLHL2 Antibody (1G7) [NBP2-02939] Analysis of extracts (35ug) from 9 different cell lines by using anti-KLHL2 monoclonal antibody.](http://images.novusbio.com/fullsize/KLHL2-Antibody-1G7-Western-Blot-NBP2-02939-img0004.jpg "Western Blot: KLHL2 Antibody (1G7) [NBP2-02939] Analysis of extracts (35ug) from 9 different cell lines by using anti-KLHL2 monoclonal antibody.")
| Reactivity | Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF |
| Clone | OTI1G7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.7 mg/ml |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1-100 and 494-593 of human KLHL2 (NP_009177) produced in HEK293T cell. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KLHL2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Theoretical MW | 65.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| Publications |
|
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.7 mg/ml |
| Purity | Immunogen affinity purified |
![Kell Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Kell Antibody [Unconjugated]")
![Immunohistochemistry Neuropilin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin1_AF566_Immunohistochemistry_21496.jpg)
![Immunohistochemistry Neuropilin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin1_AF566_Immunohistochemistry_21497.jpg)
![Western Blot Neuropilin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin1_AF566_Western_Blot_21557.jpg)
| Publication using NBP2-02939 | Applications | Species |
|---|---|---|
| Zhang E, Dong X, Chen S et al. Ubiquitin ligase KLHL2 promotes the degradation and ubiquitination of ARHGEF7 protein to suppress renal cell carcinoma progression Am J Cancer Res 2020-10-01 [PMID: 33163274] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: KLHL2 Antibody (1G7) [NBP2-02939] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KLHL2.](http://images.novusbio.com/fullsize/KLHL2-Antibody-1G7-Immunocytochemistry-Immunofluorescence-NBP2-02939-img0003.jpg "Immunocytochemistry/Immunofluorescence: KLHL2 Antibody (1G7) [NBP2-02939] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KLHL2.")
![Flow Cytometry: KLHL2 Antibody (1G7) [NBP2-02939] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KLHL2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/KLHL2-Antibody-1G7-Flow-Cytometry-NBP2-02939-img0001.jpg "Flow Cytometry: KLHL2 Antibody (1G7) [NBP2-02939] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KLHL2 antibody, and then analyzed by flow cytometry.")
![Western Blot: KLHL2 Antibody (1G7) [NBP2-02939] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLHL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLHL2.](http://images.novusbio.com/fullsize/KLHL2-Antibody-1G7-Western-Blot-NBP2-02939-img0002.jpg "Western Blot: KLHL2 Antibody (1G7) [NBP2-02939] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLHL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLHL2.")
![Western Blot Keap1 Antibody (333116) [Unconjugated]](https://images.novusbio.com/images2/Keap1_MAB3024_Western_Blot_6061.jpg)
![Simple Western Keap1 Antibody (333116) [Unconjugated]](https://images.novusbio.com/images2/Keap1_MAB3024_Simple_Western_16632.jpg)
![Knockout Validated Keap1 Antibody (333116) [Unconjugated]](https://images.novusbio.com/images2/Keap1_MAB3024_Knockout_Validated_23684.jpg)
