Ki67/MKI67 Antibody (8D5) - BSA Free

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 7 applications: ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, Immunohistochemistry, WB.

Showing Publications 1 - 10 of 34. Show All 34 Publications. Collapse Publications.

Publications using NBP2-22112	Applications	Species
Pissas G, Tziastoudi M, Divani M et Al. Malate dehydrogenase-2 inhibition shields renal tubular epithelial cells from anoxia-reoxygenation injury by reducing reactive oxygen species J Biochem Mol Toxicol 2024-09-17 [PMID: 39287333]
Zhang X, Qin Y, Chen X et Al. Clinical Value of Human Endogenous Retrovirus-H Long Terminal Repeat Associating 2 (HHLA2) in Small Cell Lung Cancer Technol Cancer Res Treat 2024-04-13 [PMID: 38613340]
Jun Seob Lee, So Young Lee, Hee Seung Chin, Na Rae Kim, Ji Won Jung Microstructure of the corneal endothelial transition zone in different laboratory animals Molecular Vision 2024-01-01 [PMID: 38601017]
Fu B, Lou Y, Lu X et Al. tRF-1:30-Gly-CCC-3 inhibits thyroid cancer via binding to PC and modulating metabolic reprogramming Life Sci Alliance 2023-12-11 [PMID: 38081642]
Meier Bürgisser G, Heuberger DM, Rieber J et al. Delineation of the healthy rabbit tongue by immunohistochemistry - A technical note Acta histochemica 2023-11-30 [PMID: 38039795] (IHC-Fr, IHC-P, Rabbit)	IHC-Fr, IHC-P	Rabbit
Celikten M, Sahin H, Senturk GE et al. The Effect of Platelet-Rich Fibrin, Platelet-Rich Plasma, and Concentrated Growth Factor in the Repair of Full Thickness Rotator Cuff Tears Journal of shoulder and elbow surgery 2023-10-26 [PMID: 37898418] (IHC-P, Rabbit)	IHC-P	Rabbit
Meier Bürgisser G, Heuberger DM, Giovanoli P et al. Delineation of the healthy rabbit tonsil by immunohistochemistry - A short communication Acta histochemica 2023-10-01 [PMID: 37804548] (Immunohistochemistry, Rabbit)	Immunohistochemistry	Rabbit
Hong Y, Chen B, Wang C et al. circPPP2R4 promotes colorectal cancer progression and reduces ROS production through the miR-646/FOXK1 axis Molecular carcinogenesis 2023-09-26 [PMID: 37750597]
Eleftheriadis T, Pissas G, Golfinopoulos S et al. Routes of Albumin Overload Toxicity in Renal Tubular Epithelial Cells International journal of molecular sciences 2023-06-01 [PMID: 37298591] (WB)	WB
Amleh A, Chen HP, Watad L et al. Arginine depletion attenuates renal cystogenesis in tuberous sclerosis complex model Cell reports. Medicine 2023-06-20 [PMID: 37290438] (IHC-P, Mouse)	IHC-P	Mouse
Liu X, Hogg GD, Zuo C et al. Context-dependent activation of STING-interferon signaling by CD11b agonists enhances anti-tumor immunity Cancer cell 2023-05-12 [PMID: 37236195]
BUrgisser GM, Heuberger DM, Schaffner N et al. Delineation of the healthy rabbit heart by immunohistochemistry - A technical note Acta histochemica 2022-12-28 [PMID: 36584538] (IHC, Rabbit)	IHC	Rabbit
Eleftheriadis T, Pissas G, Filippidis G et al. Dapagliflozin Prevents High-Glucose-Induced Cellular Senescence in Renal Tubular Epithelial Cells International journal of molecular sciences 2022-12-17 [PMID: 36555751] (WB, Human) Details: Dilution used in WB 1:1000	WB	Human
Eleftheriadis T, Pissas G, Golfinopoulos S et al. Inhibition of Malate Dehydrogenase-2 Protects Renal Tubular Epithelial Cells from Anoxia-Reoxygenation-Induced Death or Senescence Biomolecules 2022-10-03 [PMID: 36291624]
Zuo C A Thesis on Macrophage Proliferation Machinery Drives Immunosuppression and PDAC Progression Thesis 2022-01-01
Valentijn F, Knoppert S, Marquez-Exposito L et al. CCN2 aggravates acute DNA damage and the subsequent DDR-Senescence-Fibrosis sequence following renalischemia-reperfusion injury Kidney International 2022-01-01 [PMID: 35921911] (WB, WB, Human, Mouse) Details: Dilution used 1:1000	WB, WB	Human, Mouse
Wang Y, Xie C, Zhang Z et al. 3D Printed Integrated Bionic Oxygenated Scaffold for Bone Regeneration ACS applied materials & interfaces 2022-06-21 [PMID: 35729092]
Bailey T, Nieto A, McDonald P Inhibition of the Monocarboxylate Transporter 1 (MCT1) Promotes 3T3-L1 Adipocyte Proliferation and Enhances Insulin Sensitivity International journal of molecular sciences 2022-02-08 [PMID: 35163825] (ICC/IF, Mouse)	ICC/IF	Mouse
Bharti A, Kar AG, Singh D et al. Frequent promoter hypermethylation and down regulation of BNIP3: An early event during gallbladder cancer progression Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver 2022-01-26 [PMID: 35093273] (IHC-P, Human)	IHC-P	Human
Tchoukalova YD, Zacharias SRC, Mitchell N et al. Human amniotic epithelial cell transplantation improves scar remodeling in a rabbit model of acute vocal fold injury: a pilot study Stem cell research & therapy 2022-01-25 [PMID: 35073957] (IF/IHC, Rabbit)	IF/IHC	Rabbit
Everton E, Rizvi F, Smith AR Et al. Transient yet Robust Expression of Proteins in the Mouse Liver via Intravenous Injection of Lipid Nanoparticle-encapsulated Nucleoside-modified mRNA Bio-protocol 2021-10-05 [PMID: 34722830]
Eleftheriadis T, Pissas G, Filippidis G Et al. The Role of Indoleamine 2,3-Dioxygenase in Renal Tubular Epithelial Cells Senescence under Anoxia or Reoxygenation Biomolecules 2021-10-15 [PMID: 34680153] (WB, Mouse)	WB	Mouse
BUrgisser GM, Evrova O, Heuberger DM Et al. Delineation of the healthy rabbit liver by immunohistochemistry - A technical note Acta histochemica 2021-10-01 [PMID: 34627038] (IHC-P, Rabbit)	IHC-P	Rabbit
Zhang C, Li Y, Qin J et al. TMT-Based Quantitative Proteomic Analysis Reveals the Effect of Bone Marrow Derived Mesenchymal Stem Cell on Hair Follicle Regeneration Frontiers in Pharmacology 2021-06-14 [PMID: 34194323] (IHC-P, Mouse)	IHC-P	Mouse
Morson S, Yang Y, Price DJ, Pratt T Expression of Genes in the 16p11.2 Locus during Development of the Human Fetal Cerebral Cortex Cerebral cortex (New York, N.Y. : 1991) 2021-04-07 [PMID: 33825894]
Shriwas P, Roberts D, Li Y et al. A small-molecule pan-class I glucose transporter inhibitor reduces cancer cell proliferation in vitro and tumor growth in vivo by targeting glucose-based metabolism Cancer & metabolism 2021-03-26 [PMID: 33771231] (IF/IHC)	IF/IHC
Meier BUrgisser G, Heuberger DM, Giovanoli P et al. Delineation of the healthy rabbit kidney by immunohistochemistry - A technical note Acta histochemica 2021-03-07 [PMID: 33691202] (IHC-P, Rabbit)	IHC-P	Rabbit
Eleftheriadis T, Pissas G, Crespo M et al. A Role for Human Renal Tubular Epithelial Cells in Direct Allo-Recognition by CD4+ T-Cells and the Effect of Ischemia-Reperfusion International journal of molecular sciences 2021-02-09 [PMID: 33572206] (WB, Human)	WB	Human
Burgisser GM, Evrova O, Heuberger DM et al. Delineation of the healthy rabbit lung by immunohistochemistry - a technical note Acta Histochem 2020-10-29 [PMID: 33131911] (IHC-P, IHC-Fr, Rabbit)	IHC-P, IHC-Fr	Rabbit
Evrova O, BUrgisser G, EbnOther C et al. Elastic and surgeon friendly electrospun tubes delivering PDGF-BB positively impact tendon rupture healing in a rabbit Achilles tendon model Biomaterials 2020-02-01 [PMID: 31901689] (IF/IHC, Rabbit)	IF/IHC	Rabbit
Lou Q, Li Y, Hou B et al. Heat shock transcription factor 1 affects kidney tubular cell migration by regulating the TGF beta 1 Smad2/3 signaling pathway Mol Med Rep 2019-09-16 [PMID: 31545442]
Agarwal R, Liu G, Tam NW et al. Precision cell delivery in biphasic polymer systems enhances growth of keratinocytes in culture and promotes their attachment on acellular dermal matrices J Tissue Eng Regen Med 2019-02-28 [PMID: 30811860] (ICC/IF, Human)	ICC/IF	Human
Jia Z, Guo H, Xie H et al. Construction of Pedicled Smooth Muscle Tissues by Combining the Capsule Tissue and Cell Sheet Engineering Cell Transplant 2019-01-09 [PMID: 30712374] (ICC/IF, Rabbit)	ICC/IF	Rabbit
Costanza Gaetana, Doldo Elena, Ferlosio Amedeo et al. Expression and potential role of cellular retinol binding protein I in psoriasis. Oncotarget 2018-12-04 [PMID: 30613363] (IHC-P, Mouse)	IHC-P	Mouse
Show All 34 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Maxime Bourrelle-Langlois	ICC	Human	03/27/2018	View Summary Application Immunocytochemistry Sample Tested A549 human alveolar adenocarcinoma cell line Species Human Lot A-3 Comments Comments A549 human alveolar adenocarcinoma cell line, fixation with PFA 4%, blocking with PBS 1X + BSA 1% + 0.1% Tween, primary antibody used at 1:100 in blocking buffer, O/N. Secondary antibody used AlexaFluor 594, 1 hour, RT and imaged with a fluorescence microscope and counterstained with DAPI.

Array NBP2-22112

• Ki67/MKI67 Antibodies
• Ki67/MKI67 Proteins
• Ki67/MKI67 ELISA