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katanin-p80 Antibody (OTI5C4)

Images

 
Western Blot: katanin-p80 Antibody (5C4) [NBP2-01383] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY katanin-p80 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunocytochemistry/ Immunofluorescence: katanin-p80 Antibody (OTI5C4) [NBP2-01383] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY katanin-p80.
Immunohistochemistry-Paraffin: katanin-p80 Antibody (5C4) [NBP2-01383] - Staining of paraffin-embedded Human Kidney tissue using anti-katanin-p80 mouse monoclonal antibody.
Flow Cytometry: katanin-p80 Antibody (5C4) [NBP2-01383] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-katanin-p80 antibody, and ...read more
Immunohistochemistry-Paraffin: katanin-p80 Antibody (5C4) [NBP2-01383] - Staining of paraffin-embedded Human colon tissue using anti-katanin-p80 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI5C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.64 mg/ml

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katanin-p80 Antibody (OTI5C4) Summary

Immunogen
Full length human recombinant protein of human KATNB1(NP_005877) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KATNB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
72.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-01383.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.64 mg/ml
Purity
Immunogen affinity purified

Alternate Names for katanin-p80 Antibody (OTI5C4)

  • KAT
  • katanin (80 kDa)
  • katanin p80 (WD repeat containing) subunit B 1
  • katanin p80 (WD40-containing) subunit B 1
  • Katanin p80 subunit B1
  • katanin p80 WD40-containing subunit B1
  • p80 katanin

Background

Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help to organize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60 subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemble microtubules, while the p80 subunit targets the enzyme to the centrosome. Katanin is a member of the AAA family of ATPases. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for katanin-p80 Antibody (NBP2-01383)(1)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KATNB1
Entrez