Goat anti-Human Kappa Light Chain Secondary Antibody [PE/Cy5.5] Summary
Immunogen |
Purified Human Kappa light chain |
Specificity |
Based on Immunoelectrophoresis, this antibody reacts with kappa light chains on human immunoglobulins. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with mouse IgG serum proteins. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Goat |
Gene |
IGKC |
Purity |
Affinity purified |
Applications/Dilutions
Dilutions |
- ELISA
- Immunoassay
- Immunodiffusion
- Immunohistochemistry
- Immunoprecipitation
- Western Blot
|
Application Notes |
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions. |
Reactivity Notes
Based on IEP, this antibody reacts with kappa light chains on human immunoglobulins.
Packaging, Storage & Formulations
Storage |
Store at 4C in the dark. Do not freeze. |
Buffer |
PBS |
Preservative |
0.05% Sodium Azide |
Purity |
Affinity purified |
Alternate Names for Goat anti-Human Kappa Light Chain Secondary Antibody [PE/Cy5.5]
Background
Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are
guaranteed for 1 year from date of receipt.
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