![Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (KLC709) [NBP2-34259] - Formalin-fixed, paraffin-embedded human Tonsil stained with Kappa Light Chain Antibody (KLC709).](http://images.novusbio.com/fullsize/Kappa-Light-Chain-Antibody-KLC709-Immunohistochemistry-Paraffin-NBP2-34259-img0007.jpg "Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (KLC709) [NBP2-34259] - Formalin-fixed, paraffin-embedded human Tonsil stained with Kappa Light Chain Antibody (KLC709).")
| Reactivity | HuSpecies Glossary |
| Applications | Simple Western, IHC, MA |
| Clone | KLC709 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34660) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant full-length human Kappa Light Chain protein |
| Localization | Cell Surface, cytoplasmic and secreted |
| Marker | B-Cell Marker |
| Specificity | This monoclonal antibody is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. It recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | IGKC |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Theoretical MW | 22.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Kappa Light Chain Antibody (NBP2-34259)Find related products by research area.
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![Simple Western: Kappa Light Chain Antibody (KLC709) [NBP2-34259] - Simple Western lane view shows a specific band for Kappa Light Chain in 0.2 mg/ml of H. Plasma lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Kappa-Light-Chain-Antibody-KLC709-Simple-Western-NBP2-34259-img0003.jpg "Simple Western: Kappa Light Chain Antibody (KLC709) [NBP2-34259] - Simple Western lane view shows a specific band for Kappa Light Chain in 0.2 mg/ml of H. Plasma lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Simple Western: Kappa Light Chain Antibody (KLC709) [NBP2-34259] - Electropherogram image of the corresponding Simple Western lane. Kappa Light Chain antibody was used at 10 ug/ml dilution of H. Plasma lysates(s) respectively.](http://images.novusbio.com/fullsize/Kappa-Light-Chain-Antibody-KLC709-Simple-Western-NBP2-34259-img0004.jpg "Simple Western: Kappa Light Chain Antibody (KLC709) [NBP2-34259] - Electropherogram image of the corresponding Simple Western lane. Kappa Light Chain antibody was used at 10 ug/ml dilution of H. Plasma lysates(s) respectively.")
![Protein Array: Kappa Light Chain Antibody (KLC709) [NBP2-34259] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Kappa Light Chain Antibody (KLC709).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Kappa-Light-Chain-Antibody-KLC709-Protein-Array-NBP2-34259-img0008.jpg "Protein Array: Kappa Light Chain Antibody (KLC709) [NBP2-34259] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Kappa Light Chain Antibody (KLC709).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")