![Western Blot: Jagged 1 Antibody [NB600-1161] - Analysis of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only. The band at 134 kDa in lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown. Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG. Exposure time was 1 min. Predicted molecular weight is 134 kDa.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Western-Blot-NB600-1161-img0004.jpg "Western Blot: Jagged 1 Antibody [NB600-1161] - Analysis of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only. The band at 134 kDa in lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown. Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG. Exposure time was 1 min. Predicted molecular weight is 134 kDa.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This protein A purified antibody is directed against human Jagged 1 protein. The product was purified from mono-specific antiserum by affinity chromatography Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | This protein A purified Jagged 1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the N-terminus of human Jagged-1 protein. (Uniprot: P78504) |
| Localization | Type I membrane protein. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | JAG1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This product has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 150 kDa in size corresponding to Jagged-1 in mouse liver, human liver and human lung whole cell lysates. |
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| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Protein A purified |
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| Publication using NB600-1161 | Applications | Species |
|---|---|---|
| Okamoto M, Matsuda H, Joetham A et al. Jagged1 on Dendritic Cells Notch on CD4+ T Cells Initiate Lung Allergic Responsiveness by Inducing IL-4 Production. J Immunol. 2009-01-01 [PMID: 19667086] |
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![Immunocytochemistry/Immunofluorescence: Jagged 1 Antibody [NB600-1161]](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1161-img0005.jpg "Immunocytochemistry/Immunofluorescence: Jagged 1 Antibody [NB600-1161]")
![Immunohistochemistry: Jagged 1 Antibody [NB600-1161] - Human cervical cancer tissue (40X magnification) using NB600-1161 Protein A purified anti- Jagged-1 antibody. Tissue was fixed with formalin and embedded in paraffin. Hematoxylin was used to counter-stain cells. A 1:100 dilution of primary antibody was used.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunohistochemistry-NB600-1161-img0003.jpg "Immunohistochemistry: Jagged 1 Antibody [NB600-1161] - Human cervical cancer tissue (40X magnification) using NB600-1161 Protein A purified anti- Jagged-1 antibody. Tissue was fixed with formalin and embedded in paraffin. Hematoxylin was used to counter-stain cells. A 1:100 dilution of primary antibody was used.")
![Immunocytochemistry/Immunofluorescence: Jagged 1 Antibody [NB600-1161] - Human corneal epithelial cells. Primary antibody was used at a 1:500 dilution. The Jagged1 (green staining) is localized to the cytoplasm and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (blue).](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1161-img0002.jpg "Immunocytochemistry/Immunofluorescence: Jagged 1 Antibody [NB600-1161] - Human corneal epithelial cells. Primary antibody was used at a 1:500 dilution. The Jagged1 (green staining) is localized to the cytoplasm and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (blue).")
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