Jagged 1 Antibody (188331) [Alexa Fluor® 594] Summary
Immunogen |
NS0-derived recombinant human Jagged 1 Ser32-Asp296 Accession # P78504 |
Specificity |
Detects human, mouse, and rat Jagged 1. In direct ELISAs, no cross‑reactivity with recombinant human Jagged 2 is observed. Stains human Jagged 1 transfectants but not the parental cell line or human Jagged 2 transfectants in flow cytometry. |
Isotype |
IgG2b |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
JAG1 |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Flow Cytometry 0.25-1 ug/10^6 cells
|
Application Notes |
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells. |
Packaging, Storage & Formulations
Storage |
Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
Buffer |
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
Preservative |
0.09% Sodium Azide |
Concentration |
Please see the vial label for concentration. If unlisted please contact technical services. |
Notes
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Jagged 1 Antibody (188331) [Alexa Fluor® 594]
Background
Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185‑229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 shares 97% and 96% aa identity with mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1‑8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).
- Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
- Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
- Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
- Lai, E.C. (2004) Development 131:965.
- Milner, L.A. et al. (1994) Blood 83:2057.
- Nyfeler, Y. et al. (2005) EMBO J. 24:3504
-
Linheng, L. et al. (1998) Immunity 8:43.
-
Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
-
Oda, T. et al. (1997) Nat. Genet. 16:235.
-
Oda, T. et al. (1997) Genomics 43:376.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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