![Western Blot: IRGM Antibody [NBP3-18254] - Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of ~20.1 kDa IRGM protein using Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~20.1 kDa.](http://images.novusbio.com/fullsize/IRGM Antibody-Western Blot-NBP3-18254-img0004.jpg "Western Blot: IRGM Antibody [NBP3-18254] - Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of ~20.1 kDa IRGM protein using Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~20.1 kDa.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic peptide from the N-terminal of Human IRGM |
| Localization | Golgi Apparatus Membrane|Cell Membrane|Cytoplasmic Vesicle|Phagosome Membrane|Cytoplasmic Vesicle|Autophagosome Membrane|Cell Projection|Phagocytic Cup |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | IRGM |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilutions for assays should be determined by the user. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 50% glycerol |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for IRGM Antibody (NBP3-18254)Find related products by research area.
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| Gene Symbol | IRGM |
![Immunocytochemistry/Immunofluorescence: IRGM Antibody [NBP3-18254] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.](http://images.novusbio.com/fullsize/IRGM-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18254-img0001.jpg "Immunocytochemistry/Immunofluorescence: IRGM Antibody [NBP3-18254] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.")
![Immunohistochemistry: IRGM Antibody [NBP3-18254] - Immunohistochemistry analysis using Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254). Tissue: Colon Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.](http://images.novusbio.com/fullsize/IRGM-Antibody-Immunohistochemistry-NBP3-18254-img0003.jpg "Immunohistochemistry: IRGM Antibody [NBP3-18254] - Immunohistochemistry analysis using Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254). Tissue: Colon Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.")
![Immunohistochemistry: IRGM Antibody [NBP3-18254] - Immunohistochemistry analysis using Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254). Tissue: Colon Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.](http://images.novusbio.com/fullsize/IRGM-Antibody-Immunohistochemistry-NBP3-18254-img0002.jpg "Immunohistochemistry: IRGM Antibody [NBP3-18254] - Immunohistochemistry analysis using Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254). Tissue: Colon Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody (NBP3-18254) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")