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Integrin alpha L/CD11a Antibody (345908) [Unconjugated]

 
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Integrin  alpha L/CD11a was detected in immersion fixed paraffin-embedded sections of Human Spleen using Mouse Anti-Human Integrin  alpha L/CD11a Monoclonal Antibody (Catalog # MAB35952) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Integrin  alpha L/CD11a was detected in immersion fixed paraffin-embedded sections of Human Spleen using Mouse Anti-Human Integrin  alpha L/CD11a Monoclonal Antibody (Catalog # MAB35952) at 5 µg/mL ...read more
Western blot shows lysates of Jurkat Human Acute T Cell Leukemia Cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Integrin  alpha L/CD11a Monoclonal Antibody (Catalog # MAB35952) followed by ...read more
Simple Western lane view shows lysates of Human PBMCs, loaded at 0.2 mg/mL. A specific band was detected for Integrin  alpha L/CD11a at approximately 190 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clone
345908
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB35952-SP
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Integrin alpha L/CD11a Antibody (345908) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin  alpha L
Tyr26-Met1089 (Tyr660Ile)
Accession # CAA68747
Specificity
Detects human Integrin alpha L in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 50 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha L/CD11a Antibody (345908) [Unconjugated]

  • CD11 antigen-like family member A
  • CD11a antigen
  • CD11a
  • CD11Aantigen CD11A (p180), lymphocyte function-associated antigen 1, alphapolypeptide
  • Integrin alpha L
  • integrin alpha-L
  • integrin gene promoter
  • integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen1; alpha polypeptide)
  • ITGAL
  • Leukocyte adhesion glycoprotein LFA-1 alpha chain
  • Leukocyte function-associated molecule 1 alpha chain
  • LFA-1 alpha
  • LFA-1
  • LFA1A
  • LFA-1A
  • Ly-15
  • Ly-21
  • lymphocyte function-associated antigen 1

Background

Integrin subunit alpha L/CD11a is a 180 kDa type I TM glycoprotein that interacts only with Integrin  beta 2/CD18 to form LFA-1, a leukocyte adhesion protein which binds endothelial cell ICAM. Human Integrin alpha L contains a 1064 aa extracellular domain (ECD), a 20 aa TM sequence and a 58 aa cytoplasmic domain. The ECD contains seven repeats that form a beta-propeller structure and one inserted vWA domain (I domain) containing a metal ion-dependent adhesion site (MIDAS). Human and mouse Integrin alpha L ECD share 74% aa identity. A second isoform has a 53 aa insert in the ECD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Integrin alpha
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MAB35952
Integrin alpha L/CD11a Antibody (345908) [Unconjugated]
Species: Hu
Applications: WB, Simple Western, IHC

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