![Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (2B5) [NBP2-46020] - Analysis of extracts (10ug) from 2 different cell lines by using anti-IDO1 monoclonal antibody at 1:200.](http://images.novusbio.com/fullsize/Indoleamine-2-3-dioxygenase-IDO-Antibody-2B5-Western-Blot-NBP2-46020-img0010.jpg "Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (2B5) [NBP2-46020] - Analysis of extracts (10ug) from 2 different cell lines by using anti-IDO1 monoclonal antibody at 1:200.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | OTI2B5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human IDO1(NP_002155) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | IDO1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB application notes: for overexpressing samples up to a 1:4000 dilution can be used. For detecting endogenous protein, a starting dilution of 1:200 will be necessary. |
| Theoretical MW | 45.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Indoleamine 2,3-dioxygenase/IDO Antibody (NBP2-46020)Find related products by research area.
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| Gene Symbol | IDO1 |
![Immunocytochemistry/Immunofluorescence: Indoleamine 2,3-dioxygenase/IDO Antibody (2B5) [NBP2-46020] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY IDO1.](http://images.novusbio.com/fullsize/Indoleamine-2-3-dioxygenase-IDO-Antibody-2B5-Immunocytochemistry-Immunofluorescence-NBP2-46020-img0006.jpg "Immunocytochemistry/Immunofluorescence: Indoleamine 2,3-dioxygenase/IDO Antibody (2B5) [NBP2-46020] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY IDO1.")
![Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody (2B5) [NBP2-46020] - Staining of paraffin-embedded Human lymphoma tissue using anti-IDO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.](http://images.novusbio.com/fullsize/Indoleamine-2-3-dioxygenase-IDO-Antibody-2B5-Immunohistochemistry-Paraffin-NBP2-46020-img0008.jpg "Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody (2B5) [NBP2-46020] - Staining of paraffin-embedded Human lymphoma tissue using anti-IDO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.")
![Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (2B5) [NBP2-46020] - Analysis of extracts (10ug) from 2 different cell lines.](http://images.novusbio.com/fullsize/Indoleamine-2-3-dioxygenase-IDO-Antibody-2B5-Western-Blot-NBP2-46020-img0005.jpg "Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (2B5) [NBP2-46020] - Analysis of extracts (10ug) from 2 different cell lines.")
![Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (2B5) [NBP2-46020] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO.](http://images.novusbio.com/fullsize/Indoleamine-2-3-dioxygenase-IDO-Antibody-2B5-Western-Blot-NBP2-46020-img0004.jpg "Western Blot: Indoleamine 2,3-dioxygenase/IDO Antibody (2B5) [NBP2-46020] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDO.")
![Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody (2B5) [NBP2-46020] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IDO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.](http://images.novusbio.com/fullsize/Indoleamine-2-3-dioxygenase-IDO-Antibody-2B5-Immunohistochemistry-Paraffin-NBP2-46020-img0009.jpg "Immunohistochemistry-Paraffin: Indoleamine 2,3-dioxygenase/IDO Antibody (2B5) [NBP2-46020] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IDO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min.")
![Bioactivity IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFNgamma_285IF_2848.jpg)
![SDS-PAGE IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFN-gamma_285-IF_230.jpg)
![SEC-MALS IFN-gamma [Unconjugated]](https://images.novusbio.com/images/285-if_recombinant-human-ifn-gamma-protein-sec-mals-1520239228..jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![N/A IL-10 [Biotin]](https://images.novusbio.com/images2/DATA_IL10_DY417_ELISA_2014.jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")