Western Blot: Importin-8 Antibody [NBP2-24751] - Analysis of Importin-8 in HeLa lysate in the 1) absence and 2) presence of immunizing peptide using Importin-8 antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: Importin-8 Antibody [NBP2-24751] - ICC staining of HeLa cells using Importin-8 antibody at 5 ug/ml.
Simple Western: Importin-8 Antibody [NBP2-24751] - Simple Western lane view shows a specific band for Importin-8 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Immunoprecipitation reported in scientific literature (PMID 19131589)
Simple Western 1:20
Western Blot 1:200-1:2000
Application Notes
Alternative initiation produces three different forms of this protein (approximately 47, 29 and 27 kD). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Publications
Read Publications using NBP2-24751 in the following applications:
The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. This protein binds to the nuclear pore complex and, along with RanGTP and RANBP1, inhibits the GAP stimulation of the Ran GTPase. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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