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ILT3/CD85k/LILRB4 Antibody [Biotin]

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CSF monocytes express high levels of ILT3.(A) Paired blood and CSF samples were analyzed for expression of ILT3 on CD14+ monocytes by flow cytometry. The specific fluorescent index is shown. Patients were categorized according to their diagnosis as having non-inflammatory neurological diseases (NIND, n = 14; marked by dot) or inflammatory neurological disease (IND, n = 8) including 5 MS patients (marked by x) and 3 patients with other inflammatory neurological disease (diamond). A Mann-Whitney U test was performed to assess statistical significance. (B) Histogram of a representative staining in a patient with NIND. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25551576), licensed under a CC-BY license. Not internally tested by R&D Systems.
CSF monocytes express high levels of ILT3.(A) Paired blood and CSF samples were analyzed for expression of ILT3 on CD14+ monocytes by flow cytometry. The specific fluorescent index is shown. Patients were categorized ...read more
CSF monocytes express high levels of ILT3.(A) Paired blood and CSF samples were analyzed for expression of ILT3 on CD14+ monocytes by flow cytometry. The specific fluorescent index is shown. Patients were categorized ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF2425
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF2425
Carrier Free
AF2425
Carrier Free
AF2425-SP
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ILT3/CD85k/LILRB4 Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human LILRB4/CD85k/ILT3
Pro17-His257
Accession # ABM86208
Specificity
Detects human LILRB4/CD85k/ILT3 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) ILT4 and rhILT5 and 5% cross-reactivity with rhILT2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LILRB4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL
Readout System
Publications
Read Publications using
BAF2425 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ILT3/CD85k/LILRB4 Antibody [Biotin]

  • CD85 antigen-like family member K
  • CD85k antigen
  • CD85k
  • HM18
  • ILT3
  • ILT-3
  • ILT3CD85K
  • leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
  • LILRB4
  • LIR5
  • LIR5LILRB5
  • LIR-5subfamily B, member 4
  • member 4

Background

ILT3, also known as leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4) and leukocyte immunoglobulin-like receptor 5 (LIR-5), is a type I membrane protein that functions as a receptor for class I MHC antigens. It contains cytoplasmic ITIM motifs and is involved in the down-regulation of immune responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ILT3/CD85k/LILRB4 Antibody (BAF2425)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ELISA Development (Detection), Flow Cytometry, Western Blot.


Filter By Application
ELISA Development (Detection)
(1)
Flow Cytometry
(1)
Western Blot
(2)
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Filter By Species
Human
(4)
All Species
Showing Publications 1 - 4 of 4.
Publications using BAF2425 Applications Species
Waschbisch A, Sanderson N, Krumbholz M, Vlad G, Theil D, Schwab S, Maurer M, Derfuss T Interferon beta and vitamin D synergize to induce immunoregulatory receptors on peripheral blood monocytes of multiple sclerosis patients. PLoS ONE, 2014-12-31;9(12):e115488. 2014-12-31 [PMID: 25551576] (ELISA Development (Detection), Human) ELISA Development (Detection) Human
Bankey PE, Banerjee S, Zucchiatti A, De M, Sleem RW, Lin CF, Miller-Graziano CL, De AK Cytokine induced expression of programmed death ligands in human neutrophils. Immunol. Lett., 2010-02-01;129(2):100-7. 2010-02-01 [PMID: 20123111] (Flow Cytometry, Human) Flow Cytometry Human
Mori Y, Tsuji S, Inui M, Sakamoto Y, Endo S, Ito Y, Fujimura S, Koga T, Nakamura A, Takayanagi H, Itoi E, Takai T Inhibitory immunoglobulin-like receptors LILRB and PIR-B negatively regulate osteoclast development. J. Immunol., 2008-10-01;181(7):4742-51. 2008-10-01 [PMID: 18802077] (Western Blot, Human) Western Blot Human
Suciu-Foca N, Feirt N, Zhang QY, Vlad G, Liu Z, Lin H, Chang CC, Ho EK, Colovai AI, Kaufman H, D'Agati VD, Thaker HM, Remotti H, Galluzzo S, Cinti P, Rabitti C, Allendorf J, Chabot J, Caricato M, Coppola R, Berloco P, Cortesini R Soluble Ig-like transcript 3 inhibits tumor allograft rejection in humanized SCID mice and T cell responses in cancer patients. J. Immunol., 2007-06-01;178(11):7432-41. 2007-06-01 [PMID: 17513794] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol LILRB4
Uniprot