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ILK Antibody (3A9) - BSA Free

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Western Blot: ILK Antibody (3A9) [NBP2-37448] - Analysis using ILK mouse mAb against Jurkat (1), NIH3T3 (2), HeLa (3), PC-12 (4), C6 (5), COS7 (6), Raji (7), K562 (8) and MCF-7 (9) cell lysate.
Immunohistochemistry-Paraffin: ILK Antibody (3A9) [NBP2-37448] - Analysis of rectum cancer tissues using ILK mouse mAb with DAB staining.
Flow Cytometry: ILK Antibody (3A9) [NBP2-37448] - Analysis of Jurkat cells using ILK mouse mAb (green) and negative control (red).
Western Blot: ILK Antibody (3A9) [NBP2-37448] - Analysis using ILK mAb against human ILK recombinant protein. (Expected MW is 42.7 kDa)
Immunohistochemistry-Paraffin: ILK Antibody (3A9) [NBP2-37448] - Analysis of esophageal cancer tissues using ILK mouse mAb with DAB staining.
ELISA: ILK Antibody (3A9) [NBP2-37448] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, Mu, Pm, PmSpecies Glossary
Applications WB, ELISA, Flow, IHC
Clone
3A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

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Catalog# & Formulation Size Price
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ILK Antibody (3A9) - BSA Free Summary

Immunogen
Purified recombinant fragment of human ILK (AA: 97-244) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ILK
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Theoretical MW
51.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for ILK Antibody (3A9) - BSA Free

  • 59 kDa serine/threonine-protein kinase
  • DKFZp686F1765
  • EC 2.7.11.1
  • ESTM24
  • ILK
  • ILK1
  • ILK-1
  • ILK2
  • ILK-2
  • integrin-linked kinase
  • integrin-linked kinase-2
  • integrin-linked protein kinase
  • p59
  • p59ILK

Background

Integrin-linked kinase (ILK) is a key cytoplasmic plaque protein involved in the mediation of integrin functions. It plays a key role as a regulator during cell survival, cell proliferation, cell migration, and cell to cell adhesion (1). ILK links integrins to the actin cytoskeleton, allowing transduction of signals through integrins to the extracellular matrix (ECM). Also, ILK has been linked to the regulation of smooth contraction and cell motility in non-muscle cells through phosphorylation of myosin (2). Irregular activity level of ILK has been linked to expression of certain tumor. ILK overexpression caused by inactivating mutation in tumor suppressors (e.g. PTEN and APC) lead to tumor formation in transgenic mouse. Up-regulated ILK expression and activity are seen in Ewings scaromas, colorectal cancers, and late staged prostate cancers (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ILK