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IL1RAPL2 Antibody [Unconjugated]

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HepG2 human hepatocellular carcinoma cell line was stained with Goat Anti-Human IL1RAPL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1007, filled histogram) or control antibody (Catalog # AB-108-C, open ...read more
Hepa 1‑6 mouse hepatoma cell line was stained with Goat Anti-Human IL1RAPL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1007, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), ...read more
IL1RAPL2 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human IL1RAPL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1007) at 3 µg/mL for 1 hour at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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IL1RAPL2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL1RAPL2
Thr17-Glu356
Accession # Q9NP60
Specificity
Detects human IL1RAPL2 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant mouse IL1RAPL2 is observed. In Western blots, approximately 5% cross‑reactivity with recombinant human (rh) IL‑1 R7 is observed and less than 1% cross-reactivity with rhIL-1 R2 and rhIL-1 R8 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL1RAPL2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL1RAPL2 Antibody [Unconjugated]

  • IL-1R10
  • IL-1R-9
  • IL1R9IL-1 receptor accessory protein-like 2
  • IL-1R9IL-1RAPL-2
  • IL1RAPL2
  • IL-1RAPL2
  • IL-1-RAPL-2
  • IL1RAPL-2IL1RAPL-2-related protein
  • interleukin 1 receptor 9
  • interleukin 1 receptor accessory protein-like 2
  • Three immunoglobulin domain-containing IL-1 receptor-related 1
  • TIGIRR-1
  • TIGIRR-1Interleukin-1 receptor 9
  • X-linked interleukin-1 receptor accessory protein-like 2

Background

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL-1 R3), ST2 (T1/IL-1 R4), IL‑18 R alpha (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL‑18 R beta (AcPL/IL-1 R7), IL1RAPL1 (TIGIRR-2/IL-1 R8), and IL1RAPL2 (TIGIRR-1/IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL‑18BP, differ from the other members by having only one Ig domain (1). IL-1 receptor accessory protein-like 2 (IL-1 RAPL2) is alternately known as IL-1 R9 and three immunoglobulin domain containing IL-1 receptor-related molecule 1 (TIGIRR-1) and is expressed in the brain (2). Its sequence predicts an 686 amino acid (aa) residue type I transmembrane glycoprotein with a 17 aa signal peptide, a 339 aa extracellular region containing three Ig-like domains, an 18 aa transmembrane domain and a 312 aa cytoplasmic tail (3). By comparison to other IL-1 receptor family proteins, IL1RAPL2 has a C-terminal cytoplasmic extension beyond the TIR domain that is found in IL1RAPL1 and SIGIRR but not other family members (3). Human and mouse IL1RAPL2 share approximately 95% aa sequence identity. Human IL1RAPL2 is most homologous (63%) to IL1RAPL1, a receptor protein that is highly expressed in hippocampus and is involved in X-linked mental retardation (4, 5). Genes for both have been localized to human chromosome Xq22. A ligand for IL1RAPL2 has not been identified (1).

  1. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  2. Andre, R. et al. (2005) J. Neurochem. 95:324.
  3. Born, T.L. et al. (2000) J. Biol. Chem. 275:29946.
  4. Jin, H. et al. (2000) Eur. J. Hum. Genet. 8:87.
  5. Carrie, A. et al. (1999) Nat. Genet. 23:25.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IL1RAPL2
Uniprot