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Rat IL-24 ELISA Kit (Colorimetric)

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ELISA: Rat IL-24 ELISA Kit (Colorimetric) [NBP3-06782] - Samples were spiked with high concentrations of Rat IL-24 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Rat IL-24 ELISA Kit (Colorimetric) [NBP3-06782] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL

Order Details

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Rat IL-24 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat IL-24 in samples. No significant cross-reactivity or interference between Rat IL-24 and analogues was observed.
Standard Curve Range
15.63 - 1000 pg/mL
Sensitivity
9.38 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
5.35
Intra-Assay
4.53
Spike Recovery
86 - 105 %
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
IL24

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat IL-24 ELISA Kit (Colorimetric)

  • C49A
  • FISP
  • IL10B
  • IL24
  • IL-24
  • interleukin 24
  • MDA-7
  • MDA7MOB5
  • melanocyte-associated Mda-7
  • melanoma differentiation association protein 7
  • Melanoma differentiation-associated gene 7 protein
  • MOB-5
  • ST16
  • ST16interleukin-24
  • suppression of tumorigenicity 16 (melanoma differentiation)
  • Suppression of tumorigenicity 16 protein

Background

Interleukin 24 (IL-24)/melanoma differentiation-associated gene-7 (mda-7) encodes a secreted protein that exhibits significant homology to the interleukin 10 (IL-10) family of cytokines. The human IL-24 is secreted by activated peripheral blood mononuclear cells and binds two heterodimeric receptors, IL-22R1/IL-20R2 and IL-20R1/IL-20R2 resulting in the activation of JAK/STAT signaling pathways. Mda-7/IL-24, selectively induces growth suppression, apoptosis and radiosensitization in malignant gliomas in a p53-independent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol IL24