![Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Increased cytokine expression in N-WASP-null skin. Representative immunoblots and quantification of indicated proteins from epidermal lysates of 7-8-week old con and ko mice (n: 4/4, mean?+-?SD, two-tailed unpaired t-test). Representative immunoblots and quantification of indicated proteins of co-precipitations of IL-23p19 with IL-12p40 from epidermal lysates of 7-8-week-old con and ko mice (n: 4/4, mean?+-?SD, two-tailed unpaired t-test; *p?less than or equal ?0.05; **p?less than or equal ?0.01; ***p?less than or equal ?0.001) Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03704-z) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Western-Blot-NBP1-77257-img0012.jpg "Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Increased cytokine expression in N-WASP-null skin. Representative immunoblots and quantification of indicated proteins from epidermal lysates of 7-8-week old con and ko mice (n: 4/4, mean?+-?SD, two-tailed unpaired t-test). Representative immunoblots and quantification of indicated proteins of co-precipitations of IL-23p19 with IL-12p40 from epidermal lysates of 7-8-week-old con and ko mice (n: 4/4, mean?+-?SD, two-tailed unpaired t-test; *p?less than or equal ?0.05; **p?less than or equal ?0.01; ***p?less than or equal ?0.001) Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03704-z) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Eq, GpSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of mature human IL-23. The immunogen is located the last 50 amino acids of IL-23. |
| Predicted Species | Guinea Pig (93%), Equine (93%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | IL23A |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Use in immunoprecipitation reported in scientific literature (PMID: 29650963). |
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| Theoretical MW | 22 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control Peptide |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
![Immunohistochemistry IL-17D Antibody (246018) [Unconjugated]](https://images.novusbio.com/images2/IL-17D_MAB15041_Immunohistochemistry_6949.jpg)
![Intracellular Staining by Flow Cytometry IL-17D Antibody (246018) [Unconjugated]](https://images.novusbio.com/images2/IL-17D_MAB15041_Flow_Cytometry_8338.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for IL-23A/IL-23 P19 Antibody (NBP1-77257)Find related products by research area.
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![Immunohistochemistry-Paraffin: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Abdominal Aortic Aneurysm (AAA) Tissues (Validation of IL-23 in Human Abdominal Aortic Aneurysm (AAA) Tissues](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Immunohistochemistry-Paraffin-NBP1-77257-img0011.jpg "Immunohistochemistry-Paraffin: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Abdominal Aortic Aneurysm (AAA) Tissues (Validation of IL-23 in Human Abdominal Aortic Aneurysm (AAA) Tissues")
![Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Western blot analysis of IL-23 in Raji lysate with IL-23 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Western-Blot-NBP1-77257-img0003.jpg "Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Western blot analysis of IL-23 in Raji lysate with IL-23 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.")
![Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Loading: 30 ng of human IL-23 recombinant protein.Antibodies: IL-23 (4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Western-Blot-NBP1-77257-img0009.jpg "Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Loading: 30 ng of human IL-23 recombinant protein.Antibodies: IL-23 (4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Loading: 20ug of lysates per lane.Antibodies: IL-23, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Western-Blot-NBP1-77257-img0010.jpg "Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Loading: 20ug of lysates per lane.Antibodies: IL-23, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunohistochemistry: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Staining of human pancreas tissue with antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/IL-23A-IL-23-P19-Antibody-Immunohistochemistry-NBP1-77257-img0005.jpg "Immunohistochemistry: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Staining of human pancreas tissue with antibody at 5 ug/mL.")
![Western Blot: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Increased cytokine expression in N-WASP-null skin. a qRT-PCR analysis of indicated genes from back skin RNA of con & ko at indicated ages (n > = 3/3, two-tailed unpaired t-test). All normalized to GAPDH. b Representative toluidine blue staining for barrier defect in con & ko mice at indicated ages (P: E15.5 embryos used as positive controls; 2 days (2d) n: 6/5, 6 days(6d) n: 4/5, 3 independent experiments). c Representative immunofluorescence staining & quantification of IL-23p19 of con & ko back skin at indicated ages (n: 3/3, two-tailed paired t-test). Scale bar: 100 µm. d Representative sorting strategy of CD45+, alpha 6H, & alpha 6L cells from con & ko epidermal cells & qRT-PCR analysis of IL-23A & IL-17A expression in sorted cells (n: 3/3, mean ± SD, two-tailed unpaired t-test, 3 independent sortings). e Representative immunoblots & quantification of indicated proteins from epidermal lysates of 7–8-week old con & ko mice (n: 4/4, mean ± SD, two-tailed unpaired t-test). f Representative immunoblots & quantification of indicated proteins of co-precipitations of IL-23p19 with IL-12p40 from epidermal lysates of 7–8-week-old con & ko mice (n: 4/4, mean ± SD, two-tailed unpaired t-test; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001) Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03704-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77257_rabbit-polyclonal-il-23a-il-23-p19-antibody-31020241616094.jpg "Western Blot: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Increased cytokine expression in N-WASP-null skin. a qRT-PCR analysis of indicated genes from back skin RNA of con & ko at indicated ages (n > = 3/3, two-tailed unpaired t-test). All normalized to GAPDH. b Representative toluidine blue staining for barrier defect in con & ko mice at indicated ages (P: E15.5 embryos used as positive controls; 2 days (2d) n: 6/5, 6 days(6d) n: 4/5, 3 independent experiments). c Representative immunofluorescence staining & quantification of IL-23p19 of con & ko back skin at indicated ages (n: 3/3, two-tailed paired t-test). Scale bar: 100 µm. d Representative sorting strategy of CD45+, alpha 6H, & alpha 6L cells from con & ko epidermal cells & qRT-PCR analysis of IL-23A & IL-17A expression in sorted cells (n: 3/3, mean ± SD, two-tailed unpaired t-test, 3 independent sortings). e Representative immunoblots & quantification of indicated proteins from epidermal lysates of 7–8-week old con & ko mice (n: 4/4, mean ± SD, two-tailed unpaired t-test). f Representative immunoblots & quantification of indicated proteins of co-precipitations of IL-23p19 with IL-12p40 from epidermal lysates of 7–8-week-old con & ko mice (n: 4/4, mean ± SD, two-tailed unpaired t-test; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001) Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03704-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation of IL-23A/IL-23 P19 in Raji Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Raji Cells labeling IL-23A/IL-23 P19 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-77257_rabbit-polyclonal-il-23a-il-23-p19-antibody-271120241923687.jpg "Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation of IL-23A/IL-23 P19 in Raji Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Raji Cells labeling IL-23A/IL-23 P19 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).")
![Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation of IL-23A/IL-23 P19 in Raji Cells. Immunocytochemical analysis of Raji cells using anti-IL-23A/IL-23 P19 antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77257_rabbit-polyclonal-il-23a-il-23-p19-antibody-2711202419562127.jpg "Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation of IL-23A/IL-23 P19 in Raji Cells. Immunocytochemical analysis of Raji cells using anti-IL-23A/IL-23 P19 antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation of IL-23A/IL-23 P19 in Human Pancreas Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling IL-23A/IL-23 P19 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-77257_rabbit-polyclonal-il-23a-il-23-p19-antibody-2711202419392713.gif "Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation of IL-23A/IL-23 P19 in Human Pancreas Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling IL-23A/IL-23 P19 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).")
![Western Blot: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation in Human Raji Cells. Loading: 15 ug of lysates per lane.Antibodies: IL-23A/IL-23 P19 (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: Absence of blocking peptideLane B: Presence of blocking peptide.](http://images.novusbio.com/fullsize/nbp1-77257_rabbit-polyclonal-il-23a-il-23-p19-antibody-2711202419381757.jpg "Western Blot: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation in Human Raji Cells. Loading: 15 ug of lysates per lane.Antibodies: IL-23A/IL-23 P19 (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: Absence of blocking peptideLane B: Presence of blocking peptide.")
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![N/A IL-17/IL-17A [Biotin]](https://images.novusbio.com/images2/DATA_IL17_DY421_ELISA_2448.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
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![IL-9 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IL-9 [Unconjugated]")
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![SDS-PAGE IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFN-gamma_285-IF_230.jpg)
![SEC-MALS IFN-gamma [Unconjugated]](https://images.novusbio.com/images/285-if_recombinant-human-ifn-gamma-protein-sec-mals-1520239228..jpg)
![Immunohistochemistry p63/TP73L Antibody [Unconjugated]](https://images.novusbio.com/images2/p63_AF1916_Immunohistochemistry_16919.jpg)
![Immunocytochemistry p63/TP73L Antibody [Unconjugated]](https://images.novusbio.com/images2/p63_AF1916_Immunocytochemistry__Immunofluorescence_16973.jpg)
![Immunocytochemistry/ Immunofluorescence p63/TP73L Antibody [Unconjugated]](https://images.novusbio.com/images/af1916_human-p63-tp73l-affinity-purified-polyclonal-ab-41202412401237.jpg)
![SDS-PAGE IL-22 [Unconjugated]](https://images.novusbio.com/images2/IL-22_782-IL_CF_734.jpg)
![Bioactivity IL-22 [Unconjugated]](https://images.novusbio.com/images2/IL-22_782-IL_CF_743.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")