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IL-2 Antibody (JES6-1A12) [Unconjugated]

 (16 Publications)
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Recombinant Mouse IL‑2 (Catalog # 402-ML) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL‑2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑2 Monoclonal Antibody (Catalog # MAB702). The ND50 is typically 0.125-0.75 µg/mL.
Recombinant Mouse IL‑2 (Catalog # 402-ML) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications B/N, ELISA(Cap)
Clone
JES6-1A12
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB702-SP
Carrier Free
MAB702-500
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IL-2 Antibody (JES6-1A12) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse IL-2
Specificity
Detects mouse IL-2 in ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
IL2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.125-0.75 ug/mL
Application Notes
ELISA Detection: Mouse IL-2 Biotinylated Antibody (Catalog number BAF402)
Standard: Recombinant Mouse IL-2 (Catalog number 402-ML)
Publications
Read Publications using
MAB702 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-2 Antibody (JES6-1A12) [Unconjugated]

  • Aldesleukin
  • IL2
  • IL-2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • Proleukin
  • T cell growth factor
  • T-cell growth factor
  • TCGF

Background

IL-2 is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha , beta , and gamma  chains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-2 Antibody (MAB702)(16)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: Adoptive Transfer, ELISA Development, ELISpot Development, Luminex Development, Neutralization.


Filter By Application
Adoptive Transfer
(1)
ELISA Development
(1)
ELISpot Development
(1)
Luminex Development
(1)
Neutralization
(4)
All Applications
Filter By Species
Mouse
(8)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using MAB702 Applications Species
Chun-Chi Chou, Kuo-Tai Hua, Min-Wei Chen, Chin-Jui Wu, Chun-Hua Hsu, Jann-Tay Wang et al. Discovery and characterization of a monoclonal antibody targeting a conformational epitope of IL-6/IL-6R alpha to inhibit IL-6/ IL-6R alpha /gp130 hexameric signaling complex formation mAbs 12/31/2022 [PMID: 35133941]
W Piao, Y Xiong, L Li, V Saxena, KD Smith, KL Hippen, C Paluskievi, M Willsonshi, BR Blazar, R Abdi, JS Bromberg Regulatory T Cells Condition Lymphatic Endothelia for Enhanced Transendothelial Migration Cell Rep, 2020-01-28;30(4):1052-1062.e5. 1/28/2020 [PMID: 31995749]
B John, C Naczki, C Patel, A Ghoneum, S Qasem, Z Salih, N Said Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC Oncogene, 2019-02-14;0(0):. 2019-02-14 [PMID: 30765860] (Neutralization, Mouse) Neutralization Mouse
Wei Cao, Hisako Kayama, Mei Lan Chen, Amber Delmas, Amy Sun, Sang Yong Kim et al. The Xenobiotic Transporter Mdr1 Enforces T Cell Homeostasis in the Presence of Intestinal Bile Acids Immunity 2017-12-01 [PMID: 29262351]
Amaia Zabala-Letona, Amaia Arruabarrena-Aristorena, Natalia Martín-Martín, Sonia Fernandez-Ruiz, James D. Sutherland, Michelle Clasquin et al. mTORC1-dependent AMD1 regulation sustains polyamine metabolism in prostate cancer Nature 2017-07-06 [PMID: 28658205]
I Hwang, K Kim, S Choi, M Lomunova Potentiation of T Cell Stimulatory Activity by Chemical Fixation of a Weak Peptide-MHC Complex Mol. Cells, 2017-01-31;40(1):24-36. 1/31/2017 [PMID: 28152301]
Ivan D. Mascanfroni, Maisa C. Takenaka, Ada Yeste, Bonny Patel, Yan Wu, Jessica E. Kenison et al. Metabolic control of type 1 regulatory (Tr1) cell differentiation by AHR and HIF1-alpha Nature Medicine 2015-06-01 [PMID: 26005855]
Simonetta F, Gestermann N, Bloquet S, Bourgeois C Interleukin-7 optimizes FOXP3+CD4+ regulatory T cells reactivity to interleukin-2 by modulating CD25 expression. PLoS ONE, 2014-12-08;9(12):e113314. 2014-12-08 [PMID: 25485946] (Adoptive Transfer, Mouse) Adoptive Transfer Mouse
Francisco J Quintana, Hulin Jin, Evan J Burns, Meghan Nadeau, Ada Yeste, Deepak Kumar et al. Aiolos promotes TH17 differentiation by directly silencing Il2 expression Nature Immunology 2012-08-01 [PMID: 22751139]
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse) Luminex Development Mouse
Show All 16 Publications.

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Bioinformatics

Gene Symbol IL2