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IL-12/IL-35 p35 Antibody (27537) [PerCP (Peridinin-chlorophyll Protein Complex)]

Images

 
Human peripheral blood mononuclear cells (PBMCs) either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL-12/IL-35 p35 ...read more
Mouse splenocytes either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL-12/IL-35 p35 PerCP-conjugated Monoclonal Antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
27537
Clonality
Monoclonal
Host
Mouse
Conjugate
PerCP (Peridinin-chlorophyll Protein Complex)

Order Details

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IL-12/IL-35 p35 Antibody (27537) [PerCP (Peridinin-chlorophyll Protein Complex)] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35
Arg23-Ser219
Accession # P29459
Specificity
Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL12A
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publication using IC2191C.

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12/IL-35 p35 Antibody (27537) [PerCP (Peridinin-chlorophyll Protein Complex)]

  • CLMF p35
  • CLMF
  • cytotoxic lymphocyte maturation factor 1, p35
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL-12, subunit p35
  • IL-12/IL-35 p35
  • IL-12A
  • IL35 subunit
  • IL35p35
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • NF cell stimulatory factor chain 1
  • NFSK
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35

Background

Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3+ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation (2). Human FoxP3+ Tregs do not constitutively express IL-35 (3), but expression may be induced by activated dendritic cells (4).
  1. Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
  2. Collison, L.W. et al. (2007) Nature 450:566.
  3. Bardel, E. et al. (2008) J. Immunol. 181:6898.
  4. Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-12/IL-35 p35 Antibody (IC2191C)(1)


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Bioinformatics

Gene Symbol IL12A
Uniprot