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IL-12/IL-35 p35 Antibody (27537) [Janelia Fluor® 669]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, CyTOF-ready, Flow
Clone
27537
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 669

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IL-12/IL-35 p35 Antibody (27537) [Janelia Fluor® 669] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35
Arg23-Ser219
Accession # P29459
Specificity
Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL12A
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for IL-12/IL-35 p35 Antibody (27537) [Janelia Fluor® 669]

  • CLMF p35
  • CLMF
  • cytotoxic lymphocyte maturation factor 1, p35
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL-12, subunit p35
  • IL-12/IL-35 p35
  • IL-12A
  • IL35 subunit
  • IL35p35
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • NF cell stimulatory factor chain 1
  • NFSK
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35

Background

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IL12A