IL-12/IL-35 p35 Antibody (27537) [Alexa Fluor® 594] Summary
Immunogen |
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35 Arg23-Ser219 Accession # P29459 |
Specificity |
Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems. |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells
|
Packaging, Storage & Formulations
Storage |
Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
Buffer |
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
Notes
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for IL-12/IL-35 p35 Antibody (27537) [Alexa Fluor® 594]
Background
Interleukin
12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha
and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is
comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3
+
regulatory T cells and may function as an inhibitory cytokine to suppress T
cell proliferation (2). Human FoxP3
+ Tregs do not constitutively
express IL-35 (3), but expression may be induced by activated dendritic cells
(4).
- Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
- Collison, L.W. et al. (2007) Nature 450:566.
- Bardel,
E. et al. (2008) J. Immunol. 181:6898.
- Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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