Home » IKK alpha » IKK alpha Antibodies » IKK alpha Antibody [Unconjugated]

IKK alpha Antibody [Unconjugated]

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Western blot shows lysates of C2C12 mouse myoblast cell line, Rat-2 rat embryonic fibroblast cell line, and U937 human histiocytic lymphoma cell lines. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat IKKa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3768) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IKKa at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of C2C12 mouse myoblast cell line, Rat-2 rat embryonic fibroblast cell line, and U937 human histiocytic lymphoma cell lines. PVDF membrane was probed with 1 µg/mL of Sheep ...read more
IKKa was detected in immersion fixed HCT-116 human colorectal carcinoma cell line using Sheep Anti-Human/Mouse/Rat IKKa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3768) at 15 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF3768-SP
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IKK alpha Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IKK alpha
Gly375-Glu745
Accession # O15111
Specificity
Detects human, mouse, and rat IKK alpha . Does not cross-react with recombinant human (rh) IKK beta , rhIKK gamma , or rhIKK epsilon .
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CHUK
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using AF3768.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IKK alpha Antibody [Unconjugated]

  • CHUK
  • conserved helix-loop-helix ubiquitous kinaseIKBKA
  • EC 2.7.11
  • EC 2.7.11.10
  • I-kappa-B kinase 1
  • I-kappa-B kinase alpha
  • IkappaB kinase
  • IkBKA
  • IkBKalpha
  • IKK alpha
  • IKK1
  • IKK1I-kappa-B kinase-alpha
  • IKK-A
  • IKKAIkB kinase alpha subunit
  • IKK-alphainhibitor of nuclear factor kappa-B kinase subunit alpha
  • NFKBIKA
  • NFKBIKAIKK-a kinase
  • Nuclear factor NFkappaB inhibitor kinase alpha
  • Nuclear factor NF-kappa-B inhibitor kinase alpha
  • TCF16
  • TCF-16
  • Transcription factor 16

Background

I kappa B kinase alpha (IKK alpha ) is also known as IKBKA, CHUK, and IKK1. The classical active IKK complex, composed of IKK alpha , IKK beta , and two forms of processed IKK gamma , phosphorylates and inactivates I kappa B, resulting in the release and nuclear translocation of active NF-kappa B. IKK alpha contains kinase, leucine zipper, and helix-loop-helix domains, and heterodimerizes with IKK beta . NF-kappa B-inducing kinase (NIK) phosphorylates and activates IKK alpha .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IKK alpha Antibody (AF3768)(1)


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Bioinformatics

Gene Symbol CHUK
Uniprot