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IkB-beta Antibody (347428)

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Western blot shows recombinant human Ik beta -a, IkB-beta , and IkB-e (20 ng/lane). PVDF mem-brane was probed with 1 µg/mL Mouse Anti-Human/Rat IkB-beta Mono-clonal Antibody (Catalog # MAB3425) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Human/Rat IkB-beta was detected at approximately 48-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Western blot shows recombinant human Ik beta -a, IkB-beta , and IkB-e (20 ng/lane). PVDF mem-brane was probed with 1 µg/mL Mouse Anti-Human/Rat IkB-beta Mono-clonal Antibody (Catalog # MAB3425) followed by ...read more
Western blot shows lysates of MCF-7 human breast cancer cell line and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Rat IkB-beta Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB
Clone
347428
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3425-SP
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IkB-beta Antibody (347428) Summary

Immunogen
E. coli-derived recombinant human IkB-beta
Ala2-Val356
Accession # Q15653
Specificity
Detects human and rat IkB-beta in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) IkB-alpha or rhIkB-epsilon is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NFKBIB
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publication using
MAB3425 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IkB-beta Antibody (347428)

  • beta
  • IkappaBbeta
  • I-kappa-B-beta
  • ikB-B
  • IkBbeta
  • IkB-beta
  • IKBBikB-beta
  • NF-kappa-B inhibitor beta
  • NF-kappa-BIB
  • NFKBIB
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor
  • Thyroid receptor-interacting protein 9
  • TR-interacting protein 9
  • TRIP-9
  • TRIP9ikappaBbeta

Background

NF kappa B inhibitor beta (IkB-beta ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB-beta is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB-beta degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IkB-beta Antibody (MAB3425)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NFKBIB
Uniprot