![Immunohistochemistry-Paraffin: Ig gamma-4 Chain C Region Antibody (IGHG4/2042R) - Azide and BSA Free [NBP2-79850] - Formalin-fixed, paraffin-embedded human Tonsil stained with Ig gamma-4 Chain C Region Antibody (IGHG4/2042R).](http://images.novusbio.com/fullsize/Ig-gamma-4-Chain-C-Region-Antibody-IGHG4-2042R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79850-img0001.jpg "Immunohistochemistry-Paraffin: Ig gamma-4 Chain C Region Antibody (IGHG4/2042R) - Azide and BSA Free [NBP2-79850] - Formalin-fixed, paraffin-embedded human Tonsil stained with Ig gamma-4 Chain C Region Antibody (IGHG4/2042R).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC, MA |
| Clone | IGHG4/2042R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified by Protein A Column. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79716). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant full-length human Ig gamma-4 Chain C Region protein (Uniprot: P01861) |
| Localization | Cytoplasmic |
| Marker | G4m Marker |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | IGHG4 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 150 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | IGHG4 |
![Protein Array: Ig gamma-4 Chain C Region Antibody (IGHG4/2042R) - Azide and BSA Free [NBP2-79850] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Ig gamma-4 Chain C Region Antibody (IGHG4/2042R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in](http://images.novusbio.com/fullsize/Ig-gamma-4-Chain-C-Region-Antibody-IGHG4-2042R-Azide-and-BSA-Free-Protein-Array-NBP2-79850-img0002.jpg "Protein Array: Ig gamma-4 Chain C Region Antibody (IGHG4/2042R) - Azide and BSA Free [NBP2-79850] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Ig gamma-4 Chain C Region Antibody (IGHG4/2042R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in")
![Control Mouse IgG3 Isotype Control (133316) [Unconjugated]](https://images.novusbio.com/images2/IgG3_MAB007_Control_19774.jpg)
![Control Goat IgG Isotype Control [Unconjugated]](https://images.novusbio.com/images2/IgG_AB108C_Control_19376.jpg)
![Immunocytochemistry/ Immunofluorescence Goat IgG Isotype Control [Unconjugated]](https://images.novusbio.com/images/ab-108-c_normal-goat-igg-control-8220249504611.jpg)
![Immunocytochemistry/ Immunofluorescence Goat IgG Isotype Control [Unconjugated]](https://images.novusbio.com/images/ab-108-c_normal-goat-igg-control-8220249505997.jpg)
![Simple Western alpha 2-Macroglobulin Antibody [Unconjugated]](https://images.novusbio.com/images2/alpha_2Macroglobulin_AF1938_Simple_Western_20705.jpg)
![Western Blot alpha 2-Macroglobulin Antibody [Unconjugated]](https://images.novusbio.com/images2/alpha_2Macroglobulin_AF1938_Western_Blot_20706.jpg)
![Immunocytochemistry alpha 2-Macroglobulin Antibody [Unconjugated]](https://images.novusbio.com/images2/alpha_2Macroglobulin_AF1938_Immunocytochemistry__Immunofluorescence_17394.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")