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IFT122 Antibody

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Immunocytochemistry/ Immunofluorescence: IFT122 Antibody [NBP2-97508] - Immunofluorescence staining of IFT122 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% ...read more
Immunohistochemistry-Paraffin: IFT122 Antibody [NBP2-97508] - Immunochemical staining of human IFT122 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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IFT122 Antibody Summary

Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Immunogen
Produced in rabbits immunized with E. coli-derived Human IFT122 fragment.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IFT122
Purity
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Proclin 300
Purity
Antigen and protein A Affinity-purified

Alternate Names for IFT122 Antibody

  • intraflagellar transport 122 homolog (Chlamydomonas)
  • intraflagellar transport protein 122 homolog
  • SPGWD repeat-containing protein 140
  • WD repeat domain 10
  • WD repeat-containing protein 10
  • WDR10WDR10p
  • WDR140CED

Background

IFT122 is a gene that codes for a protein necessary for cilia formation during neuronal patterning, as it recruits TULP3 to primary cilia and works as a negative regulator of Shh signaling. IFT122 has five isoforms, with lengths of 1241, 1242, 1182, 1131, and 1292 amino acids and weights of 142, 142, 135, 129, and 147 kDa respectively. Current studies are being done on several diseases and disorders including Japanese spotted fever, endemic typhus, vasomer rhinitis, trigeminal neuralgia, synostosis, brachydactyly, paraplegia, neuronitis, and hepatitis. IFT122 has also been shown to have interactions with UBC, IQCB1, and ORF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol IFT122