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IFN-gamma Antibody (K3.53) [Janelia Fluor® 669]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, ELISA, ELISA(Cap), ELISA(Det), Flow
Clone
K3.53
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 669

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC2852U-100UG
Alexa Fluor 405
IC2852V-100UG
Alexa Fluor 488
IC2852G-100UG
Alexa Fluor 532
FAB2852X
Alexa Fluor 594
IC2852T-100UG
Alexa Fluor 647
IC2852R-100UG
Alexa Fluor 700
IC2852N-100UG
Alexa Fluor 750
IC2852S-100UG
Allophycocyanin
FAB2852A
Biotin
FAB2852B
CoraFluor 1
FAB2852CL1
DyLight 350
FAB2852D
DyLight 405
FAB2852E
DyLight 488
FAB2852K
DyLight 550
FAB2852L
DyLight 594
FAB2852M
DyLight 650
FAB2852W
DyLight 680
FAB2852Y
DyLight 755
FAB2852Z
FITC
FAB2852F
HRP
FAB2852H
Janelia Fluor 525
FAB2852JF525
Janelia Fluor 549
FAB2852I
Janelia Fluor 585
FAB2852JF585
Janelia Fluor 635
FAB2852JF635
Janelia Fluor 646
FAB2852J
PE
FAB2852P
PerCP
FAB2852C
Unconjugated
MAB2852-100
mFluor Violet 450 SE
FAB2852MFV450
mFluor Violet 500 SE
FAB2852MFV500
mFluor Violet 610 SE
FAB2852MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB2852-100
Carrier Free
MAB2852-SP
Carrier Free
MAB2852-500
Carrier Free
MAB2852-100
Carrier Free
IC2852V-100UG
Carrier Free
IC2852U-100UG
Carrier Free
IC2852T-100UG
Carrier Free
IC2852S-100UG
Carrier Free
IC2852R-100UG
Carrier Free
IC2852N-100UG
Carrier Free
IC2852G-100UG
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IFN-gamma Antibody (K3.53) [Janelia Fluor® 669] Summary

Immunogen
E. coli-derived recombinant human IFN-gamma
Specificity
Detects human IFN‑ gamma in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse IFN‑ gamma , recombinant rat IFN‑ gamma , recombinant porcine IFN‑ gamma , recombinant feline IFN‑ gamma , or recombinant cotton rat IFN‑ gamma is observed.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
IFNG
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • ELISA Standard (Matched Pair)
  • Intracellular Staining by Flow Cytometry
  • Neutralization
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for IFN-gamma Antibody (K3.53) [Janelia Fluor® 669]

  • IFG
  • IFI
  • IFNG
  • IFNgamma
  • IFN-gamma
  • Immune interferon
  • interferon gamma
  • interferon, gamma

Background

IFN-gamma, a type II interferon and an acid-labile interferon, is mainly produced by CD4 and CD8 T-lymphocytes and natural killer cells activated by antigens, mitogens or alloantigens. IFN-gamma shows no significant homology with IFN-beta or IFN-alpha. IFN-gamma has antiviral and antiparasitic activities and exhibits also immunoregulatory and proinflammatory activities. IFN-gamma induces cytokine production and promotes antigen presentation by up regulating the expression of MHC proteins as well as other antigen proteins. In contrast to other interferons, which are mostly antiviral, the main activity of IFN-gamma seems to be immunomodulatory. Human IFN-gamma is species-specific and is only biologically active in human and primate cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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IFN-gamma Antibody
(K3.53) [Janelia Fluo ...
FAB2852JF669
IFN-gamma Antibody (K3.53) [Janelia Fluor® 669]
Species: Hu
Applications: WB, B/N, ELISA, ELISA(Cap), ELISA(Det), Flow

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IFN-gamma Antibody (FAB2852JF669). (Showing 1 - 1 of 1 FAQ).

  1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
    • We are research use only.

Secondary Antibodies

 

Isotype Controls

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Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.

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Bioinformatics

Gene Symbol IFNG