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ICAM-1/CD54 Antibody [Unconjugated]

 (12 Publications)
 (1 Review)
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Western blot shows lysates of HUVEC human umbilical vein endothelial cells and Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 24 hours. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human ICAM-1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF720) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ICAM-1/CD54 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells and Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 24 hours. ...read more
ICAM-1/CD54 was detected in immersion fixed A431 human epithelial carcinoma cell line (left panel; positive stain) and A549 human lung carcinoma cell line (right panel; negative stain) using Sheep Anti-Human ICAM-1/CD54 ...read more
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and human ICAM-1/CD54 Ramos human Burkitt's lymphoma cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human ICAM‑1/CD54 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, ICC/IF, KO
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF720
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF720-SP
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ICAM-1/CD54 Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ICAM-1
Extracellular domain
Specificity
Selected for its ability to block the adhesion of HSB‑2 cells to immobilized recombinant human (rh) ICAM‑1. In direct ELISAs, approximately 5% cross-reactivity with rhICAM-3 is observed and less than 2% cross-reactivity with rhICAM-2, rhICAM-4, rhICAM-5, recombinant rat ICAM‑1, recombinant mouse ICAM‑1, and rhVCAM-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ICAM1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.20 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Adhesion Blockade
  • Immunocytochemistry 1-15 ug/mL
  • Knockout Validated 0.2 ug/mL
  • Western Blot 0.2 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF720 in the following applications:
Publications
Read Publications using
AF720 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAM-1/CD54 Antibody [Unconjugated]

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma , TNF-alpha , IL-1 beta and LPS. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ICAM-1/CD54 Antibody (AF720)(12)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC, Neutralization, Western Blot.


Filter By Application
ICC
(2)
Neutralization
(5)
Western Blot
(1)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF720 Applications Species
Patricia Himmels, Thi Thu Thao Nguyen, Maresa Caunt Mitzner, Alfonso Arrazate, Stacey Yeung, Jeremy Burton et al. T cell‐dependent bispecific antibodies alter organ‐specific endothelial cell–T cell interaction EMBO reports 3/6/2023 [PMID: 36621885]
R Taftaf, X Liu, S Singh, Y Jia, NK Dashzeveg, AD Hoffmann, L El-Shennaw, EK Ramos, V Adorno-Cru, EJ Schuster, D Scholten, D Patel, Y Zhang, AA Davis, C Reduzzi, Y Cao, P D'Amico, Y Shen, M Cristofani, WA Muller, V Varadan, H Liu ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer Nature Communications, 2021-08-11;12(1):4867. 2021-08-11 [PMID: 34381029] (Neutralization, Human) Neutralization Human
Rivera-Serrano EE, Gonzalez-Lopez O, Das A, Lemon SM. Cellular entry and uncoating of naked and quasi-enveloped human hepatoviruses Elife 2/25/2019 [PMID: 30801249]
Multiplexed, targeted profiling of single-cell proteomes and transcriptomes in a single reaction Genome Biol, 2016-09-19;17(1):188. 2016-09-19 [PMID: 27640647] (ICC, Human) ICC Human
Antony Prabhu, Bhaswati Sarcar, C. Ryan Miller, Sung-Hak Kim, Ichiro Nakano, Peter Forsyth et al. Ras-mediated modulation of pyruvate dehydrogenase activity regulates mitochondrial reserve capacity and contributes to glioblastoma tumorigenesis Neuro-Oncology 9/1/2015 [PMID: 25712957]
Jose Javier Miguel-Hidalgo, James C. Overholser, George J. Jurjus, Herbert Y. Meltzer, Lesa Dieter, Lisa Konick et al. Vascular and extravascular immunoreactivity for intercellular adhesion molecule 1 in the orbitofrontal cortex of subjects with major depression: Age-dependent changes Journal of Affective Disorders 8/1/2011 [PMID: 21536333]
Hashizume M, Mihara M High molecular weight hyaluronic acid inhibits IL-6-induced MMP production from human chondrocytes by up-regulating the ERK inhibitor, MKP-1. Biochem. Biophys. Res. Commun., 2010-11-06;403(2):184-9. 2010-11-06 [PMID: 21059338] (Neutralization, Human) Neutralization Human
Filip Kukulski, Fethia Ben Ben Yebdri, Fariborz Bahrami, Michel Fausther, Alain Tremblay, Jean Sévigny Endothelial P2Y2 receptor regulates LPS-induced neutrophil transendothelial migration in vitro Molecular Immunology 2/1/2010 [PMID: 20022380]
Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes. J. Leukoc. Biol., 2008-06-30;84(4):1109-19. 2008-06-30 [PMID: 18591415] (Neutralization, Human) Neutralization Human
Schellerer VS, Croner RS, Weinlander K, Hohenberger W, Sturzl M, Naschberger E Endothelial cells of human colorectal cancer and healthy colon reveal phenotypic differences in culture. Lab. Invest., 2007-09-17;87(11):1159-70. 2007-09-17 [PMID: 17876298] (ICC, Human) ICC Human
Show All 12 Publications.

Review for ICAM-1/CD54 Antibody (AF720) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF720:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 Flow Human 01/09/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 20022380
SpeciesHuman

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Sheep IgG Isotype Control [Unconjugated]
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TNF-alpha [Unconjugated]
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Recent Reviews

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1
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 Verified Customer
01/09/2015
Application: Flow
Species: Human
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Bioinformatics

Gene Symbol ICAM1