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ICAM-1/CD54 Antibody (MEM-111) - BSA Free

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Western Blot: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - ICAM-1 protein expression levels in rat lung tissue after the different treatments in each group. Immunoblotting showed increase in ICAM-1 after one course of ...read more
Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Flow cytometry analysis (surface staining) of CD54 expression in activated human peripheral blood with anti-CD54 (MEM-111) PE
Western Blot: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Of CD54 expression in activated (A) and nonactivated (B) Huvec cells by antibody MEM-111. Lower bands represent tubulin as a loading control.
Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Analysis using the FITC conjugate of NB500-318. Staining of Human peripheral blood lymphocytes with MEM-111 FITC.
Flow Cytometry: ICAM-1/CD54 Antibody (MEM-111) [NB500-318] - Analysis using PE conjugate of NB500-318. A cell surface stain was performed on THP-1 cells with ICAM-1/CD54 antibody (MEM-111) NB500-318 (blue) and a matched ...read more

Product Details

Summary
Reactivity Hu, Rt, BvSpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready
Clone
MEM-111
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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ICAM-1/CD54 Antibody (MEM-111) - BSA Free Summary

Additional Information
Clone MEM-111 was used by HLDA to establish CD designation.
Immunogen
Raji human Burkitt's lymphoma cell line (NM_000201.2).
Specificity
The antibody MEM-111 reacts with an extracellular epitope CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells. HLDA VI; WS Code AS A049
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ICAM1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:2000
  • Flow Cytometry 0.5-4 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
WB: Use under non-reducing conditions. Use in IHC-Frozen reported in scientific literature (PMID 26256764). This antibody is CyTOF ready.
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
ICAM-1/CD54 Overexpression Lysate
Publications
Read Publications using
NB500-318 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.9% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for ICAM-1/CD54 Antibody (MEM-111) - BSA Free

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

CD54 (ICAM1) is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase in inflammation. Also, ICAM1 is detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ICAM-1/CD54 Antibody (NB500-318)(7)

We have publications tested in 3 confirmed species: Human, Rat, Bovine.

We have publications tested in 4 applications: IF/IHC, IHC-Fr, IHC-P, WB.


Filter By Application
IF/IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
WB
(2)
All Applications
Filter By Species
Human
(1)
Rat
(4)
Bovine
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB500-318 Applications Species
Chung NPY, Khan KMF, Kaner RJ et al. HIV induces airway basal progenitor cells to adopt an inflammatory phenotype Scientific reports 2021-02-17 [PMID: 33597552] (WB, Human) WB Human
Carlos CP, Silva AA, Gil CD, Oliani SM. Pharmacological treatment with galectin-1 protects against renal ischaemia-reperfusion injury Sci Rep 2018-06-22 [PMID: 29934646] (IHC-P, Rat) IHC-P Rat
Thomas A, Ou-Yang HD, Lowe-Krentz L. Biomimetic channel modeling local vascular dynamics of pro-inflammatory endothelial changes. Biomicrofluidics. 2016-01-06 [PMID: 26858813]
Liu WJ, Zhong ZJ, Cao LH et al. Paclitaxel-induced lung injury and its amelioration by parecoxib sodium. Sci Rep. 2015-08-10 [PMID: 26256764] (IHC-Fr, Rat) IHC-Fr Rat
Xiang S, Dong NG, Liu JP et al. Inhibitory effects of suppressor of cytokine signaling 3 on inflammatory cytokine expression and migration and proliferation of IL-6/IFN-gamma-induced vascular smooth muscle cells. J Huazhong Univ Sci Technolog Med Sci. 2013-10-01 [PMID: 24142708] (WB, Rat) WB Rat
Ng CY, Kamisah Y, Faizah O, Jaarin K. The role of repeatedly heated soybean oil in the development of hypertension in rats: association with vascular inflammation Int J Exp Pathol 2012-10-01 [PMID: 22974219] (IF/IHC, Rat) IF/IHC Rat
Rosano JM, Tousi N, Scott RC et al. A physiologically realistic in vitro model of microvascular networks. Biomed Microdevices 2009-10-01 [PMID: 19452279] (Bovine) Bovine

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for ICAM-1/CD54 Antibody (NB500-318). (Showing 1 - 3 of 3 FAQs).

  1. Is the antibody kappa chain specific?
    • Our product NB500-318 is isotype IgG2a kappa.
  2. The vial says to store at -20C but the data sheet says to store at 4C. Which is correct?
    • The correct storage temperature for NB500-318 is 4C.
  3. Could you tell me which epitope recognizes NB500-318 antibody? I'm looking for some anti-equine antibody, but there is none, so I would like to compare epitope sequence of your anti-bovine antibody and horse ICAM1 sequence.
    • Unfortunately, we do not routinely epitope map our products. At this time, we do not have any information as to the exact epitope recognized by NB500-318. However, this is a licensed clone, and you may be able to find some publication information with information for which you are looking. We currently have 4 listed publications for this product.Also, a publication search on the clone may reveal some further information. If you are interested in testing this in equine, we have a program that may interest you. Please see below. If you would like to test an antibody in an untested species and/or application and share your results with us, I can recommend our Innovator's Reward™ program. Our Innovator’s Reward™ program was created to allow researchers the opportunity to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply submit an online review detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product.
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Bioinformatics

Gene Symbol ICAM1