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ICAM-1/CD54 Antibody (BBIG-I1 (11C81)) [Unconjugated]

Name: ICAM-1/CD54 Antibody (BBIG-I1 (11C81)) [Unconjugated]
Brand: Novus Biologicals
SKU: BBA3
Rating: 4.8 (4 reviews)

(81 Publications)

(4 Reviews)

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ICAM‑1/CD54 was detected in live unfixed C32 human melanoma cell line using Mouse Anti-Human ICAM‑1/CD54 Monoclonal Antibody (Catalog # BBA3) at 5 µg/mL for 1 hour at 2° - 8° C. Cells were ...read more

Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human ICAM-1/CD54 Monoclonal Antibody (Catalog # BBA3) or (B) Mouse IgG1 Isotype Control (MAB002) followed by anti-Mouse IgG ...read more

Western blot shows lysates of C32. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ICAM‑1/CD54 Monoclonal Antibody (Catalog # BBA3) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody ...read more

Product Details

Summary

Reactivity	HuSpecies Glossary
Applications	WB, Flow, IP, B/N, ICC/IF
Clone	BBIG-I1 (11C81)
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Concentration	LYOPH

Order Details

View Available Conjugates

Catalog# & Conjugate	Size	Price
Biotin BBA9

Fluorescein BBA20

View Available Formulations

Catalog# & Formulation	Size	Price
Carrier Free BBA20

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Conjugate	Catalog #	Availability	Size	Price
Biotin	BBA9
Fluorescein	BBA20

ICAM-1/CD54 Antibody (BBIG-I1 (11C81)) [Unconjugated] Summary

Immunogen	Activated HUVEC human umbilical vein endothelial cells
Specificity	This antibody was selected for its ability to stain human ICAM-1/CD54 transfected COS cells. It does not stain COS cells transfected with human E-Selectin, VCAM-1, or PECAM-1.
Source	N/A
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	ICAM1
Purity Statement	Protein A or G purified from hybridoma culture supernatant
Endotoxin Note	<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

Adhesion Blockade
Flow Cytometry 0.25 ug/10^6 cells
Immunocytochemistry 5-25 ug/mL
Immunoprecipitation
Western Blot 2 ug/mL

Reviewed Applications

Read 4 Reviews rated 4.8

using
BBA3 in the following applications:

Immunofluorescence
Immunocytochemistry

Publications

Read Publications using
BBA3 in the following applications:

Bead-based Bioassay
1 publication
Bioassay
2 publications
Cell-based ELISA
1 publication
ELISA Capture
1 publication
ELISA Development
1 publication
ELISA Development (Capture)
1 publication
Flow Cytometry
8 publications
Functional Assay
2 publications
ICC
14 publications
IF
1 publication
IHC
4 publications
IHC-Fr
1 publication
Immunocytochemistry
2 publications
Immunoprecipitation
2 publications
Inhibition
1 publication
Neutralization
16 publications
Western Blot
8 publications

Packaging, Storage & Formulations

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAM-1/CD54 Antibody (BBIG-I1 (11C81)) [Unconjugated]

BB2
CD54 antigen
CD54
cell surface glycoprotein P3.58
human rhinovirus receptor
ICAM1
ICAM-1
intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
intercellular adhesion molecule 1
Major group rhinovirus receptor
P3.58

Background

ICAM-1 is a member of the immunoglobulin superfamily whose expression is upregulated on leukocytes, epithelial cells and resting endothelial cells in response to inflammatory signals. ICAM-1 binds the leukocyte integrins LFA-1 and Mac-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ICAM-1/CD54 Antibody (BBA3)(81)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 17 applications: Bead-based Bioassay, Bioassay, Cell-based ELISA, ELISA Capture, ELISA Development, ELISA Development (Capture), Flow Cytometry, Functional Assay, ICC, IF, IHC, IHC-Fr, Immunocytochemistry, Immunoprecipitation, Inhibition, Neutralization, Western Blot.

Showing Publications 1 - 10 of 81. Show All 81 Publications.

Publications using BBA3	Applications	Species
Liang, NW;Wilson, C;Davis, B;Wolf, I;Qyli, T;Moy, J;Beebe, DJ;Schnapp, LM;Kerr, SC;Faust, HE; Modeling lung endothelial dysfunction in sepsis-associated ARDS using a microphysiological system Physiological reports 2024-07-01 [PMID: 38981846] (Immunocytochemistry, Human)	Immunocytochemistry	Human
Chien, Y;Huang, XY;Yarmishyn, AA;Chien, CS;Liu, YH;Hsiao, YJ;Lin, YY;Lai, WY;Huang, SC;Lee, MS;Chiou, SH;Yang, YP;Chiou, GY; Paracrinal regulation of neutrophil functions by coronaviral infection in iPSC-derived alveolar type II epithelial cells Virus research 2024-07-01 [PMID: 38754785] (Neutralization, Human)	Neutralization	Human
Cacho-Navas, C;López-Pujante, C;Reglero-Real, N;Colás-Algora, N;Cuervo, A;Conesa, JJ;Barroso, S;de Rivas, G;Ciordia, S;Paradela, A;D'Agostino, G;Manzo, C;Feito, J;Andrés, G;Molina-Jiménez, F;Majano, P;Correas, I;Carazo, JM;Nourshargh, S;Huch, M;Millán, J; ICAM-1 nanoclusters regulate hepatic epithelial cell polarity by leukocyte adhesion-independent control of apical actomyosin eLife 2024-04-10 [PMID: 38597186] (Immunoprecipitation, Immunocytochemistry, Human)	Immunoprecipitation, Immunocytochemistry	Human
Kintos, DP;Salagiannis, K;Sgouros, A;Nikolaropoulos, SS;Topouzis, S;Fousteris, MA; Identification of new multi-substituted 1H-pyrazolo[3,4-c]pyridin-7(6H)-ones as soluble guanylyl cyclase (sGC) stimulators with vasoprotective and anti-inflammatory activities Bioorganic chemistry 2024-02-02 [PMID: 38335755] (ELISA Capture, Rat)	ELISA Capture	Rat
Howard, C;Joof, F;Hu, R;Smith, JD;Zheng, Y; Probing cerebral malaria inflammation in 3D human brain microvessels Cell reports 2023-10-10 [PMID: 37819760] (ICC, Human)	ICC	Human
Marlene Geyer, Lisa-Marie Gaul, Sabrina Luigia D`Agosto, Vincenzo Corbo, Karla Queiroz The tumor stroma influences immune cell distribution and recruitment in a PDAC-on-a-chip model Frontiers in Immunology 2023-05-02 [PMID: 37205118]
Haley Ehlers, Arnaud Nicolas, Frederik Schavemaker, Jeroen P. M. Heijmans, Martin Bulst, Sebastiaan J. Trietsch et al. Vascular inflammation on a chip: A scalable platform for trans-endothelial electrical resistance and immune cell migration Frontiers in Immunology 2023-01-24 [PMID: 36761747]
Orianne Constant, Ghizlane Maarifi, Jonathan Barthelemy, Marie-France Martin, Bachirou Tinto, Giovanni Savini et al. Differential effects of Usutu and West Nile viruses on neuroinflammation, immune cell recruitment and blood–brain barrier integrity Emerging Microbes & Infections 2023-12-31 [PMID: 36495563]
C Akoto, A Willis, CF Banas, JA Bell, D Bryant, C Blume, DE Davies, EJ Swindle IL-33 Induces an Antiviral Signature in Mast Cells but Enhances Their Permissiveness for Human Rhinovirus Infection Viruses, 2022-11-01;14(11):. 2022-11-01 [PMID: 36366528] (Neutralization, Human)	Neutralization	Human
Victor Delprat, Camille Huart, Olivier Feron, Fabrice Soncin, Carine Michiels The impact of macrophages on endothelial cells is potentiated by cycling hypoxia: Enhanced tumor inflammation and metastasis Frontiers in Oncology 2022-09-28 [PMID: 36248978]
Iosif Pediaditakis, Konstantia R. Kodella, Dimitris V. Manatakis, Christopher Y. Le, Sonalee Barthakur, Alexander Sorets et al. A microengineered Brain-Chip to model neuroinflammation in humans iScience 2022-08-19 [PMID: 35982785]
Andrea Kosta, Abdelilah Mekhloufi, Lorenzo Lucantonio, Alessandra Zingoni, Alessandra Soriani, Marco Cippitelli et al. GAS6/TAM signaling pathway controls MICA expression in multiple myeloma cells Frontiers in Immunology 2022-07-28 [PMID: 35967396]
W Singhatana, S Kitpakorns, M Toso, P Pavasant IFNgamma-primed periodontal ligament cells regulate T-cell responses via IFNgamma-inducible mediators and ICAM-1-mediated direct cell contact Royal Society open science, 2022-07-27;9(7):220056. 2022-07-27 [PMID: 35911203] (Neutralization, Human)	Neutralization	Human
Cristina Cacho-Navas, Natalia Reglero-Real, Natalia Colás-Algora, Susana Barroso, Gema de Rivas, Kostantinos Stamatakis et al. Plasmolipin regulates basolateral-to-apical transcytosis of ICAM-1 and leukocyte adhesion in polarized hepatic epithelial cells Cellular and Molecular Life Sciences 2022-01-09 [PMID: 34999972]
Lilian Schimmel, Keng Yih Chew, Claudia J Stocks, Teodor E Yordanov, Patricia Essebier, Arutha Kulasinghe et al. Endothelial cells are not productively infected by SARS‐CoV‐2 Clinical & Translational Immunology 2021-10-24 [PMID: 34721846]
Izabela K. Piechocka, Sarah Keary, Alberto Sosa-Costa, Lukas Lau, Nitin Mohan, Jelena Stanisavljevic et al. Shear forces induce ICAM-1 nanoclustering on endothelial cells that impact on T-cell migration Biophysical Journal 2021-07-01 [PMID: 34087211]
O Tong, G Duette, TR O'Neil, CM Royle, H Rana, B Johnson, N Popovic, S Dervish, MAE Brouwer, H Baharlou, E Patrick, G Ctercteko, S Palmer, E Lee, E Hunter, AN Harman, AL Cunningham, N Nasr Plasmacytoid dendritic cells have divergent effects on HIV infection of initial target cells and induce a pro-retention phenotype PloS Pathogens, 2021-04-19;17(4):e1009522. 2021-04-19 [PMID: 33872331] (Neutralization, Human)	Neutralization	Human
E Russo, P Runge, NH Jahromi, H Naboth, A Landtwing, R Montecchi, N Leicht, MC Hunter, Y Takai, C Halin CD112 Regulates Angiogenesis and T Cell Entry into the Spleen Cells, 2021-01-15;10(1):. 2021-01-15 [PMID: 33467729] (Bioassay, Human)	Bioassay	Human
Dafeng Yang, Stefan Haemmig, Haoyang Zhou, Daniel Pérez-Cremades, Xinghui Sun, Lei Chen et al. Methotrexate attenuates vascular inflammation through an adenosine-microRNA-dependent pathway eLife 2021-01-08 [PMID: 33416495]
Masahiro Takeuchi, Paula T Vidigal, Mateus T Guerra, Melanie A Hundt, Marie E Robert, Maria Olave-Martinez et al. Neutrophils interact with cholangiocytes to cause cholestatic changes in alcoholic hepatitis Gut 2020-11-19 [PMID: 33214166]
V Simion, H Zhou, JB Pierce, D Yang, S Haemmig, Y Tesmenitsk, G Sukhova, PH Stone, P Libby, MW Feinberg LncRNA VINAS regulates atherosclerosis by modulating NF-&kappaB and MAPK signaling JCI Insight, 2020-11-05;0(0):. 11/5/2020 [PMID: 33021969]
H Israelov, O Ravid, D Atrakchi, D Rand, S Elhaik, Y Bresler, R Twitto-Gre, L Omesi, S Liraz-Zalt, F Gosselet, M Schnaider, I Cooper Caspase-1 has a critical role in blood-brain barrier injury and its inhibition contributes to multifaceted repair J Neuroinflammation, 2020-09-09;17(1):267. 2020-09-09 [PMID: 32907600] (ICC, Western Blot, Human)	ICC, Western Blot	Human
M Clé, C Desmetz, J Barthelemy, MF Martin, O Constant, G Maarifi, V Foulongne, K Bolloré, Y Glasson, F De Bock, M Blaquiere, L Dehouck, N Pirot, E Tuaillon, S Nisole, F Najioullah, P Van de Per, A Cabié, N Marchi, F Gosselet, Y Simonin, S Salinas Zika Virus Infection Promotes Local Inflammation, Cell Adhesion Molecule Upregulation, and Leukocyte Recruitment at the Blood-Brain Barrier MBio, 2020-08-04;11(4):. 2020-08-04 [PMID: 32753493] (Neutralization, Human)	Neutralization	Human
Y Chen, D Tang, L Zhu, T Yuan, Y Jiao, H Yan, W Yu hnRNPA2/B1 Ameliorates LPS-Induced Endothelial Injury through NF-&kappaB Pathway and VE-Cadherin/&beta-Catenin Signaling Modulation In Vitro Mediators Inflamm., 2020-05-30;2020(0):6458791. 2020-05-30 [PMID: 32565727] (Western Blot, Human)	Western Blot	Human
Hideaki Nishihara, Sasha Soldati, Adrien Mossu, Maria Rosito, Henriette Rudolph, William A. Muller et al. Human CD4+ T cell subsets differ in their abilities to cross endothelial and epithelial brain barriers in vitro Fluids and Barriers of the CNS 2020-12-01 [PMID: 32008573]
SP Davies, GM Reynolds, AL Wilkinson, X Li, R Rose, M Leekha, YS Liu, R Gandhi, E Buckroyd, J Grove, NM Barnes, RC May, SG Hubscher, DH Adams, Y Huang, O Qureshi, Z Stamataki Hepatocytes Delete Regulatory T Cells by Enclysis, a CD4+ T Cell Engulfment Process Cell Rep, 2019-11-05;29(6):1610-1620.e4. 2019-11-05 [PMID: 31693899] (IF, Human)	IF	Human
Carine Poussin, Bart Kramer, Henriette L Lanz, Angelique Van den Heuvel, Alexandra Laurent, Thomas Olivier et al. 3D human microvessel-on-a-chip model for studying monocyte-to-endothelium adhesion under flow – application in systems toxicology ALTEX 2019-01-01 [PMID: 31445503]
P Guo, J Yang, D Liu, L Huang, G Fell, J Huang, MA Moses, DT Auguste Dual complementary liposomes inhibit triple-negative breast tumor progression and metastasis Sci Adv, 2019-03-20;5(3):eaav5010. 2019-03-20 [PMID: 30906868] (Neutralization, Human)	Neutralization	Human
MR Blatchley, F Hall, S Wang, HC Pruitt, S Gerecht Hypoxia and matrix viscoelasticity sequentially regulate endothelial progenitor cluster-based vasculogenesis Sci Adv, 2019;5(3):eaau7518. 2019 [PMID: 30906859] (ICC, Mouse)	ICC	Mouse
Jaeyoung Cho, Hyuck-Se Kweon, Sung-Oh Huh, Ali Sadra Augmented reduction in colonic inflammatory markers of dextran sulfate sodium-induced colitis with a combination of 5-aminosalicylic acid and AD-lico™ from Glycyrrhiza inflata Animal Cells and Systems 2018-06-02 [PMID: 30460097]
G Ecklu-Mens, RW Olsen, A Bengtsson, MF Ofori, L Hviid, ATR Jensen, Y Adams Blood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes PLoS ONE, 2018-10-09;13(10):e0204177. 2018-10-09 [PMID: 30300360] (Flow Cytometry, ICC, Human)	Flow Cytometry, ICC	Human
E Raschi, CB Chighizola, L Cesana, D Privitera, F Ingegnoli, C Mastaglio, PL Meroni, MO Borghi Immune complexes containing scleroderma-specific autoantibodies induce a profibrotic and proinflammatory phenotype in skin fibroblasts Arthritis Res. Ther., 2018-08-29;20(1):187. 2018-08-29 [PMID: 30157947] (Inhibition, Human)	Inhibition	Human
Bing Liu, Lili Xu, Xinming Yu, Wei Li, Xiaozhi Sun, Shun Xiao, Mingjin Guo, Haofu Wang Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF??. Molecular Medicine Reports, 2018-06-20;0(0):1791-3004. 2018-06-20 [PMID: 29956764] (Western Blot, Human)	Western Blot	Human
Q Su, Y Sun, Z Ye, H Yang, B Kong, L Li Pinocembrin protects endothelial cells from oxidized LDL-induced injury Cytokine, 2018-06-18;0(0):. 2018-06-18 [PMID: 29914794] (Western Blot, Human)	Western Blot	Human
J Weiler, M Mohr, KS Zänker, T Dittmar Matrix metalloproteinase-9 (MMP9) is involved in the TNF-Î±-induced fusion of human M13SV1-Cre breast epithelial cells and human MDA-MB-435-pFDR1 cancer cells Cell Commun. Signal, 2018-04-10;16(1):14. 2018-04-10 [PMID: 29636110] (Immunoprecipitation, Neutralization, Human)	Immunoprecipitation, Neutralization	Human
J Kroon, A Schaefer, J van Rijsse, M Hoogenboez, F van Alphen, P Hordijk, ESG Stroes, S Strömblad, J van Rheene, JD van Buul Inflammation-Sensitive Myosin-X Functionally Supports Leukocyte Extravasation by Cdc42-Mediated ICAM-1-Rich Endothelial Filopodia Formation J. Immunol., 2018-01-31;0(0):. 2018-01-31 [PMID: 29386254] (Bead-based Bioassay, ICC, Human)	Bead-based Bioassay, ICC	Human
Chang-Yun Liu, Zhen-Hua Zhao, Zhi-Ting Chen, Chun-Hui Che, Zhang-Yu Zou, Xiao-Min Wu et al. DL-3-n-butylphthalide protects endothelial cells against advanced glycation end product-induced injury by attenuating oxidative stress and inflammation responses Experimental and Therapeutic Medicine 2017-09-01 [PMID: 28962149]
Malara N, Trunzo V, Musolino G, Aprigliano S, Rotta G, Macrina L, Limongi T, Gratteri S, Di Fabrizio E, Renzulli A, Fini M, Mollace V Soluble CD54 induces human endothelial cells ex vivo expansion useful for cardiovascular regeneration and tissue engineering application. Int J Cardiol Heart Vasc, 2015-01-09;6(0):48-53. 2015-01-09 [PMID: 28785626] (Neutralization, Human)	Neutralization	Human
A Schaefer, TJ van Duijn, J Majolee, K Burridge, PL Hordijk Endothelial CD2AP Binds the Receptor ICAM-1 To Control Mechanosignaling, Leukocyte Adhesion, and the Route of Leukocyte Diapedesis In Vitro J. Immunol., 2017-05-08;0(0):. 2017-05-08 [PMID: 28484055] (Functional Assay, ICC, Human)	Functional Assay, ICC	Human
P Guo, J Yang, DR Bielenberg, D Dillon, D Zurakowski, MA Moses, DT Auguste A quantitative method for screening and identifying molecular targets for nanomedicine J Control Release, 2017-03-22;0(0):. 2017-03-22 [PMID: 28341549] (ICC, Human)	ICC	Human
Nicolas Espagnolle, Adélie Balguerie, Emmanuelle Arnaud, Luc Sensebé, Audrey Varin CD54-Mediated Interaction with Pro-inflammatory Macrophages Increases the Immunosuppressive Function of Human Mesenchymal Stromal Cells Stem Cell Reports 2017-04-01 [PMID: 28330617]
Ruth Lyck, Marc-André Lécuyer, Michael Abadier, Christof B Wyss, Christoph Matti, Maria Rosito et al. ALCAM (CD166) is involved in extravasation of monocytes rather than T cells across the blood–brain barrier Journal of Cerebral Blood Flow & Metabolism 2017-08-01 [PMID: 28273717]
J Arebro, S Ekstedt, E Hjalmarsso, O Winqvist, S Kumlien Ge, LO Cardell A possible role for neutrophils in allergic rhinitis revealed after cellular subclassification Sci Rep, 2017-03-08;7(0):43568. 2017-03-08 [PMID: 28272395] (Flow Cytometry, Human)	Flow Cytometry	Human
Minu Karthika Ganesan 3D co-culture model to analyze the crosstalk between endothelial and smooth muscle cells Tissue Eng Part C Methods, 2017-01-01;0(0):. 2017-01-01 [PMID: 27923320] (ICC, Human)	ICC	Human
Gilli Galore-Haskel, Yael Nemlich, Eyal Greenberg, Shira Ashkenazi, Motti Hakim, Orit Itzhaki et al. A novel immune resistance mechanism of melanoma cells controlled by the ADAR1 enzyme Oncotarget 2015-08-21 [PMID: 26338962]
Zhang P, Fu C, Hu Y, Dong C, Song Y, Song E C6-ceramide nanoliposome suppresses tumor metastasis by eliciting PI3K and PKCzeta tumor-suppressive activities and regulating integrin affinity modulation. Sci Rep, 2015-03-20;5(0):9275. 2015-03-20 [PMID: 25792190] (Flow Cytometry, Human)	Flow Cytometry	Human
Mukherjee S, Kim J, Mooren O, Shahan S, Cohan M, Cooper J Role of cortactin homolog HS1 in transendothelial migration of natural killer cells. PLoS ONE, 2015-02-27;10(2):e0118153. 2015-02-27 [PMID: 25723543] (IHC, Human)	IHC	Human
Céline Tellier, Déborah Desmet, Laurenne Petit, Laure Finet, Carlos Graux, Martine Raes et al. Cycling Hypoxia Induces a Specific Amplified Inflammatory Phenotype in Endothelial Cells and Enhances Tumor-Promoting Inflammation In Vivo12 Neoplasia 2015-01-23 [PMID: 25622900]
Onken , Michael, Li , Jinmei, Cooper , John A Uveal melanoma cells utilize a novel route for transendothelial migration. PLoS ONE, 2014-12-15;9(12):e115472. 2014-12-15 [PMID: 25506912] (Neutralization, Human)	Neutralization	Human
Mooren O, Li J, Nawas J, Cooper J Endothelial cells use dynamic actin to facilitate lymphocyte transendothelial migration and maintain the monolayer barrier. Mol Biol Cell, 2014-10-29;25(25):4115-29. 2014-10-29 [PMID: 25355948] (Bioassay, Human)	Bioassay	Human
Roe , Kelsey, Orillo , Beverly, Verma , Saguna West Nile virus-induced cell adhesion molecules on human brain microvascular endothelial cells regulate leukocyte adhesion and modulate permeability of the in vitro blood-brain barrier model. PLoS ONE, 2014-07-18;9(7):e102598. 2014-07-18 [PMID: 25036379] (Neutralization, Human)	Neutralization	Human
Spilioti E, Jaakkola M, Tolonen T, Lipponen M, Virtanen V, Chinou I, Kassi E, Karabournioti S, Moutsatsou P Phenolic acid composition, antiatherogenic and anticancer potential of honeys derived from various regions in Greece. PLoS ONE, 2014-04-21;9(4):e94860. 2014-04-21 [PMID: 24752205] (ELISA Development (Capture), Human)	ELISA Development (Capture)	Human
Noviello M, Tedesco F, Bondanza A, Tonlorenzi R, Rosaria Carbone M, Gerli M, Marktel S, Napolitano S, Cicalese M, Ciceri F, Peretti G, Cossu G, Bonini C Inflammation converts human mesoangioblasts into targets of alloreactive immune responses: implications for allogeneic cell therapy of DMD. Mol Ther, 2014-04-16;22(7):1342-52. 2014-04-16 [PMID: 24736278] (Neutralization, Human)	Neutralization	Human
Ohnuma , Kiyoshi, Fujiki , Ayaka, Yanagihara , Kana, Tachikawa , Saoko, Hayashi , Yohei, Ito , Yuzuru, Onuma , Yasuko, Chan , Techuan, Michiue , Tatsuo, Furue , Miho K, Asashima , Makoto Enzyme-free passage of human pluripotent stem cells by controlling divalent cations. Sci Rep, 2014-04-11;4(0):4646. 2014-04-11 [PMID: 24721898] (IHC, Human)	IHC	Human
Park , Su-Jung, Kim , Ki-Jo, Kim , Wan-Uk, Cho , Chul-Soo Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor. J Immunol, 2014-02-26;192(7):3003-10. 2014-02-26 [PMID: 24574497] (Neutralization, Human)	Neutralization	Human
Gakhar , Gunjan, Navarro , Vicente, Jurish , Madelyn, Lee , Guang Yu, Tagawa , Scott T, Akhtar , Naveed H, Seandel , Marco, Geng , Yue, Liu , He, Bander , Neil H, Giannakakou , Paraskev, Christos , Paul J, King , Michael, Nanus , David M Circulating tumor cells from prostate cancer patients interact with E-selectin under physiologic blood flow. PLoS ONE, 2013-12-27;8(12):e85143. 12/27/2013 [PMID: 24386459]
Lin , Yi-Wen, Huang , Chun-Yao, Chen , Yung-Hsi, Shih , Chun-Min, Tsao , Nai-Wen, Lin , Cheng-Ye, Chang , Nen-Chun, Tsai , Chien-Su, Tsai , Hsiao-Ya, Tsai , Jui-Chi, Huang , Po-Hsun, Li , Chi-Yuan, Lin , Feng-Yen GroEL1, a heat shock protein 60 of Chlamydia pneumoniae, impairs neovascularization by decreasing endothelial progenitor cell function. PLoS ONE, 2013-12-23;8(12):e84731. 2013-12-23 [PMID: 24376840] (Western Blot, Human)	Western Blot	Human
Lauridsen H, Pober J, Gonzalez A A composite model of the human postcapillary venule for investigation of microvascular leukocyte recruitment. FASEB J, 2013-12-02;28(3):1166-80. 2013-12-02 [PMID: 24297702] (ICC, Human)	ICC	Human
Schellerer V, Langheinrich M, Hohenberger W, Croner R, Merkel S, Rau T, Sturzl M, Naschberger E Tumor-associated fibroblasts isolated from colorectal cancer tissues exhibit increased ICAM-1 expression and affinity for monocytes. Oncol Rep, 2013-11-20;31(1):255-61. 2013-11-20 [PMID: 24253852] (ICC, IHC-Fr, Human)	ICC, IHC-Fr	Human
Tsai S, Huang P, Peng Y, Chang W, Tsai H, Leu H, Chen J, Lin S Zoledronate attenuates angiotensin II-induced abdominal aortic aneurysm through inactivation of Rho/ROCK-dependent JNK and NF-kappaB pathway. Cardiovasc Res, 2013-12-01;100(3):501-10. 2013-12-01 [PMID: 24225494] (Western Blot, Mouse)	Western Blot	Mouse
Dunyaporn Trachootham, Gang Chen, Wan Zhang, Weiqin Lu, Hui Zhang, Jinsong Liu et al. Loss of p53 in stromal fibroblasts promotes epithelial cell invasion through redox-mediated ICAM1 signal Free Radical Biology and Medicine 2013-05-01 [PMID: 23376231]
Macrophages induce differentiation of plasma cells through CXCL10/IP-10. J. Exp. Med., 2012-09-17;209(10):1813-23. 2012-09-17 [PMID: 22987802] (Neutralization, Human)	Neutralization	Human
Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes. J Biol Chem, 2012-06-15;287(32):26478-94. 2012-06-15 [PMID: 22707717] (Cell-based ELISA, Human)	Cell-based ELISA	Human
Kimberly M. Stroka, Irena Levitan, Helim Aranda-Espinoza OxLDL and substrate stiffness promote neutrophil transmigration by enhanced endothelial cell contractility and ICAM-1 Journal of Biomechanics 2012-06-26 [PMID: 22560286]
Kevin Wilhelmsen, Kailin R Mesa, Arun Prakash, Fengyun Xu, Judith Hellman Activation of endothelial TLR2 by bacterial lipoprotein upregulates proteins specific for the neutrophil response Innate Immunity 2012-08-01 [PMID: 22186927]
Stroka KM, Aranda-Espinoza H Endothelial cell substrate stiffness influences neutrophil transmigration via myosin light chain kinase-dependent cell contraction. Blood, 2011-06-07;118(6):1632-40. 2011-06-07 [PMID: 21652678] (ICC, Human)	ICC	Human
Yue Zhao, Deborah A. Howatt, Florence Gizard, Takashi Nomiyama, Hannes M. Findeisen, Elizabeth B. Heywood et al. Deficiency of the NR4A Orphan Nuclear Receptor NOR1 Decreases Monocyte Adhesion and Atherosclerosis Circulation Research 2010-08-20 [PMID: 20558821]
Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes. J. Immunol., 2010-03-31;184(9):4880-8. 2010-03-31 [PMID: 20357258] (Neutralization, Human)	Neutralization	Human
Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4. J. Hepatol., 2009-07-30;51(4):734-49. 2009-07-30 [PMID: 19703720] (IHC, Neutralization, Human)	IHC, Neutralization	Human
Rajesh M, Mukhopadhyay P, Batkai S, Hasko G, Liaudet L, Drel VR, Obrosova IG, Pacher P Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption. Am. J. Physiol. Heart Circ. Physiol., 2007-03-23;293(1):H610-9. 2007-03-23 [PMID: 17384130] (Western Blot, Human)	Western Blot	Human
Bae E, Kim WJ, Kang YM, Suk K, Koh EM, Cha HS, Ahn KS, Huh TL, Lee WH Glucocorticoid-induced tumour necrosis factor receptor-related protein-mediated macrophage stimulation may induce cellular adhesion and cytokine expression in rheumatoid arthritis. Clin. Exp. Immunol., 2007-03-15;148(3):410-8. 2007-03-15 [PMID: 17359498] (Flow Cytometry, Human)	Flow Cytometry	Human
Kelly KA, Allport JR, Yu AM, Sinh S, Sage EH, Gerszten RE, Weissleder R SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration. J. Leukoc. Biol., 2006-12-18;81(3):748-56. 2006-12-18 [PMID: 17178915] (Functional Assay, Human)	Functional Assay	Human
Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils. Arthritis Rheum., 2006-07-01;54(7):2096-108. 2006-07-01 [PMID: 16802344] (Neutralization, Human)	Neutralization	Human
Ramachandran R, Morice AH, Compton SJ Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts. Am. J. Respir. Cell Mol. Biol., 2006-02-23;35(1):133-41. 2006-02-23 [PMID: 16498082] (Flow Cytometry, Human)	Flow Cytometry	Human
Amin MA, Haas CS, Zhu K, Mansfield PJ, Kim MJ, Lackowski NP, Koch AE Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB. Blood, 2005-11-29;107(6):2252-61. 2005-11-29 [PMID: 16317091] (ICC, Western Blot, Human)	ICC, Western Blot	Human
Domeij H, Modeer T, Quezada HC, Yucel-Lindberg T Cell expression of MMP-1 and TIMP-1 in co-cultures of human gingival fibroblasts and monocytes: the involvement of ICAM-1. Biochem. Biophys. Res. Commun., 2005-11-02;338(4):1825-33. 2005-11-02 [PMID: 16288711] (Flow Cytometry, Human)	Flow Cytometry	Human
Tamai R, Asai Y, Ogawa T Requirement for intercellular adhesion molecule 1 and caveolae in invasion of human oral epithelial cells by Porphyromonas gingivalis. Infect. Immun., 2005-10-01;73(10):6290-8. 2005-10-01 [PMID: 16177300] (Flow Cytometry, Human)	Flow Cytometry	Human
Frattini J, Kudo FA Differential effects of orbital and laminar shear stress on endothelial cells. J. Vasc. Surg., 2005-05-01;41(5):869-80. 2005-05-01 [PMID: 15886673] (ICC, Human)	ICC	Human
Yamamoto H, Nagata M, Sakamoto Y CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1. Ann. Allergy Asthma Immunol., 2005-02-01;94(2):292-300. 2005-02-01 [PMID: 15765748] (ELISA Development, Human)	ELISA Development	Human
Helantera I, Loginov R, Koskinen P, Tornroth T, Gronhagen-Riska C, Lautenschlager I Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts. Nephrol. Dial. Transplant., 2005-02-16;20(4):790-6. 2005-02-16 [PMID: 15716293] (IHC, Human)	IHC	Human
Almenar-Queralt A, Duperray A, Miles LA, Felez J, Altieri DC Apical topography and modulation of ICAM-1 expression on activated endothelium. Am. J. Pathol., 1995-11-01;147(5):1278-88. 1995-11-01 [PMID: 7485391] (Flow Cytometry, ICC, Human)	Flow Cytometry, ICC	Human
Show All 81 Publications. Collapse Publications.

Reviews for ICAM-1/CD54 Antibody (BBA3) (4) 4.84

Average Rating: 4.8

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01/20/2015

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Application	Immunofluorescence
Sample Tested	See PMID: 21277303
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Application	Immunocytochemistry
Sample Tested	See PMID: 23104645
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Application	Immunofluorescence
Sample Tested	See PMID: 21164106
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Application	Immunofluorescence
Sample Tested	See PMID: 22560286
Species	Human

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Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human ICAM-1/CD54 Fluorescein-conjugated Monoclonal Antibody (Catalog # BBA20) or (B) Mouse IgG1Fluorescein Isotype Control (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.

ICAM-1/CD54 Antibody (BBIG-I1 (11C81)) [Fluorescein]
BBA20

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.

TNF-alpha [Unconjugated]
210-TA-005

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	Verified Customer 01/20/2015
Application:	IF
Species:	Human

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Application:	ICC
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ICAM1

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

ICAM-1/CD54 Antibody (BBIG-I1 (11C81)) [Unconjugated]

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Publications for ICAM-1/CD54 Antibody (BBA3)(81)

Reviews for ICAM-1/CD54 Antibody (BBA3) (4) 4.84

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