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ICAM-1/CD54 Antibody (14C11) [Unconjugated]

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ICAM-1/CD54 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human ICAM-1/CD54 Monoclonal Antibody (Catalog # MAB720) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
ICAM-1/CD54 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human ICAM-1/CD54 Monoclonal Antibody (Catalog # MAB720) at 25 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Cap), ICC/IF
Clone
14C11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB720-SP
Carrier Free
MAB720-500
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ICAM-1/CD54 Antibody (14C11) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ICAM-1
Extracellular domain
Specificity
Detects recombinant human (rh)ICAM-1 in direct ELISAs and Western blots. In Western blots, shows no cross-reactivity with rrICAM-1, rhICAM-2, rhICAM-3, rhVCAM-1, and rmDCC.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ICAM1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 5-25 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Human ICAM-1/CD54 Biotinylated Antibody (Catalog number BAF720)
Standard: Recombinant Human ICAM-1/CD54 (Catalog number ADP4)
Publications
Read Publications using
MAB720 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAM-1/CD54 Antibody (14C11) [Unconjugated]

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be upregulated upon cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICAM-1/CD54 Antibody (14C11) [Unconjugated]
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Publications for ICAM-1/CD54 Antibody (MAB720)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ELISA Development, ELISA Microarray Development, Luminex Development.


Filter By Application
ELISA Development
(1)
ELISA Microarray Development
(1)
Luminex Development
(1)
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Filter By Species
Human
(3)
All Species
Showing Publications 1 - 5 of 5.
Publications using MAB720 Applications Species
I Gomez, B Ward, C Souilhol, C Recarti, M Ariaans, J Johnston, A Burnett, M Mahmoud, LA Luong, L West, M Long, S Parry, R Woods, C Hulston, B Benedikter, C Niespolo, R Bazaz, S Francis, E Kiss-Toth, M van Zandvo, A Schober, P Hellewell, PC Evans, V Ridger Neutrophil microvesicles drive atherosclerosis by delivering miR-155 to atheroprone endothelium Nat Commun, 2020-01-10;11(1):214. 1/10/2020 [PMID: 31924781]
Adukpo , Selorme, Kusi , Kwadwo A, Ofori , Michael, Tetteh , John K A, Amoako-Sakyi , Daniel, Goka , Bamenla, Adjei , George O, Edoh , Dominic, Akanmori , Bartholo, Gyan , Ben A, Dodoo , Daniel High plasma levels of soluble intercellular adhesion molecule (ICAM)-1 are associated with cerebral malaria. PLoS ONE, 2013-12-27;8(12):e84181. 2013-12-27 [PMID: 24386348] (ELISA Development, Human) ELISA Development Human
Stephanie Traub, Alexandra Nikonova, Alan Carruthers, Rebecca Dunmore, Katherine A. Vousden, Leila Gogsadze et al. An Anti-Human ICAM-1 Antibody Inhibits Rhinovirus-Induced Exacerbations of Lung Inflammation PLoS Pathogens 8/1/2013 [PMID: 23935498]
Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (ELISA Microarray Development, Human) ELISA Microarray Development Human
Pfleger C, Schloot N, ter Veld F Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J. Immunol. Methods, 2007-10-22;329(1):214-8. 2007-10-22 [PMID: 17964594] (Luminex Development, Human) Luminex Development Human

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Bioinformatics

Gene Symbol ICAM1