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Human Phospho-HGFR/c-MET DuoSet IC ELISA, 2 Plate

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The human epidermoid carcinoma cell line, A431, was treated with 100 ng/mL recombinant human HGF for five minutes to induce tyrosine phosphorylation of HGF R. Serial dilutions of lysates were analyzed by (A) IP-Western blot and (B) this ELISA. IPs were done using an anti-HGF R polyclonal antibody and Protein G agarose. Immunoblots were incubated with a biotinylated anti-phosphotyrosine monoclonal antibody (Catalog # BAM1676) to detect phospho-HGF R (p-HGF R). Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by chemiluminescent detection using WesternGloTM Chemiluminescent Detection Substrate (Catalog # AR004).
The human epidermoid carcinoma cell line, A431, was treated with 100 ng/mL recombinant human HGF for five minutes to induce tyrosine phosphorylation of HGF R. Serial dilutions of lysates were analyzed by (A) IP-Western ...read more
A431 cells were untreated or treated with 100 ng/mL recombinant human HGF for five minutes. ELISA and IP-Western blot (inset) analyses were done using 50 μg and 500 μg of lysate, respectively. IP-Western blots for ...read more
A431 cells were treated with 100 ng/mL recombinant human HGF for five minutes. The indicated amounts of recombinant extracellular domains of human HGF R (Catalog #358-MT), human EGF R (Catalog #1095-ER) or human IGF-I ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
Biotin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
DYC2480-5
Biotin
DYC2480E
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Human Phospho-HGFR/c-MET DuoSet IC ELISA, 2 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
MET

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DYC2480-2.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Phospho-HGFR/c-MET DuoSet IC ELISA, 2 Plate

  • AUTS9
  • cMET
  • c-MET
  • EC 2.7.10
  • EC 2.7.10.1
  • hepatocyte growth factor receptor
  • HGF R
  • HGF receptor
  • HGF/SF receptor
  • HGFR
  • Met (c-Met)
  • met proto-oncogene (hepatocyte growth factor receptor)
  • met proto-oncogene tyrosine kinase
  • MET
  • oncogene MET
  • Proto-oncogene c-Met
  • RCCP2
  • Scatter factor receptor
  • SF receptor
  • Tyrosine-protein kinase Met

Background

HGF receptor, a product of the proto-oncogene c-met, is a heterodimeric transmembrane glycoprotein that is a receptor-type tyrosine kinase. c-MET is synthesized as a single-chain precursor (pr170) which undergoes post-translational glycosylation and proteolytic cleavage to give rise to the heterodimeric mature form.

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Human
Phospho-HGFR/c-MET Du ...
DYC2480-2
Human Phospho-HGFR/c-MET DuoSet IC ELISA, 2 Plate
Species: Hu
Applications: ELISA

Publications for HGFR/c-MET (DYC2480-2)(5)

We have publications tested in 1 confirmed species: Human.


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Human
(5)
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Showing Publications 1 - 5 of 5.
Publications using DYC2480-2 Applications Species
M Su, B Qin, F Liu, Y Chen, R Zhang Andrographolide enhanced 5-fluorouracil-induced antitumor effect in colorectal cancer via inhibition of c-MET pathway Drug Des Devel Ther, 2017-11-23;11(0):3333-3341. 2017-11-23 [PMID: 29200829] (Human) Human
Fu , Le, Guo , Wei, Liu , Bingshan, Sun , Linlin, Bi , Zhenghon, Zhu , Li, Wang , Xinyan, Liu , Bin, Xie , Qian, Li , Ke Shedding of c-Met ectodomain correlates with c-Met expression in non-small cell lung cancer. Biomarkers, 2013-03-01;18(2):126-35. 2013-03-01 [PMID: 23410046] (Human) Human
Xu L, Nilsson MB, Saintigny P, Cascone T, Herynk MH, Du Z, Nikolinakos PG, Yang Y, Prudkin L, Liu D, Lee JJ, Johnson FM, Wong KK, Girard L, Gazdar AF, Minna JD, Kurie JM, Wistuba II, Heymach JV Epidermal growth factor receptor regulates MET levels and invasiveness through hypoxia-inducible factor-1alpha in non-small cell lung cancer cells. Oncogene, 2010-02-15;29(18):2616-27. 2010-02-15 [PMID: 20154724] (Human) Human
Uhland K, Siphos B, Arkona C, Schuster M, Petri B, Steinmetzer P, Mueller F, Schweinitz A, Steinmetzer T, Van De Locht A Use of IHC and newly designed matriptase inhibitors to elucidate the role of matriptase in pancreatic ductal adenocarcinoma. Int. J. Oncol., 2009-08-01;35(2):347-57. 2009-08-01 [PMID: 19578749] (Human) Human
Phin S, Marchand-Adam S, Fabre A, Marchal-Somme J, Bantsimba-Malanda C, Kataoka H, Soler P, Crestani B Imbalance in the pro-hepatocyte growth factor activation system in bleomycin-induced lung fibrosis in mice. Am. J. Respir. Cell Mol. Biol., 2009-05-15;42(3):286-93. 2009-05-15 [PMID: 19448157] (Human) Human

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Bioinformatics

Gene Symbol MET