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Human Phospho-ErbB3/Her3 DuoSet IC ELISA, 2 Plate

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The human breast cancer cell line, MDA-MB-453, was treated with 100 ng/mL recombinant HRG-beta 1 (Catalog # 396-HB) for five minutes to induce tyrosine phosphorylation of ErbB3. Serial dilutions of lysates were analyzed by R&D Systems’ Phospho-ErbB3 DuoSet IC ELISA (Catalog # DYC1769) and by IP-Western blot (inset). For IP-Westerns, IPs were done using anti-ErbB3 monoclonal antibody and anti-mouse IgG agarose. Immunoblots were incubated with biotinylated anti-phosphotyrosine monoclonal antibody (Cat # BAM1676) to detect phospho-ErbB3. Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by ECL detection. ErbB3 was observed at its predicted size (inset; 185 kDa, arrow) in Western blot analysis.
The human breast cancer cell line, MDA-MB-453, was treated with 100 ng/mL recombinant HRG-beta 1 (Catalog # 396-HB) for five minutes to induce tyrosine phosphorylation of ErbB3. Serial dilutions of lysates were ...read more
MDA-MB-453 cells were untreated or treated with 100 ng/mL of recombinant HRG-beta 1 (Catalog # 396-HB) for five minutes. Cell lysates (100 μg) were analyzed by R&D Systems’ Phospho-ErbB3 DuoSet IC ELISA (Catalog # ...read more
MDA-MB-453 cells were treated with 100 ng/mL of recombinant HRG-beta 1 (Catalog # 396-HB) for five minutes. The indicated amounts of ErbB3 (Catalog # 348-ER), ErbB2 (Catalog # 1129-ER) or EGF R (Catalog # 1095-ER) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
Biotin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
DYC1769-5
Biotin
DYC1769E
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Human Phospho-ErbB3/Her3 DuoSet IC ELISA, 2 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
ERBB3

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DYC1769-2.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Phospho-ErbB3/Her3 DuoSet IC ELISA, 2 Plate

  • c-erbB3
  • EC 2.7.10
  • EC 2.7.10.1
  • ErbB3
  • ErbB-3
  • erbB3-S
  • HER3
  • HER3c-erbB-3
  • LCCS2
  • lethal congenital contracture syndrome 2
  • MDA-BF-1
  • MGC88033
  • p180-ErbB3
  • p45-sErbB3
  • p85-sErbB3
  • Proto-oncogene-like protein c-ErbB-3
  • receptor tyrosine-protein kinase erbB-3
  • Tyrosine kinase-type cell surface receptor HER3
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)

Background

ErbB3, also called Her3 (human epidermal growth factor receptor 3) in humans, is a type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB3 binds neuregulins NRG1 and NRG2, but lacks a working kinase domain; heterodimerization with another ErbB receptor is required for signaling. ErbB3 is important in development of Schwann cells, neural crest cells, heart valves and the sympathetic nervous system. When expressed with ErbB2 in breast, skin, ovary and gastrointestinal tumors, ErbB3 is a potent mitogen.

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Human
Phospho-ErbB3/Her3 Du ...
DYC1769-2
Human Phospho-ErbB3/Her3 DuoSet IC ELISA, 2 Plate
Species: Hu
Applications: ELISA

Publications for ErbB3/Her3 (DYC1769-2)(4)

We have publications tested in 1 confirmed species: Human.


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Bioinformatics

Gene Symbol ERBB3