Home » IL-17/IL-17A » IL-17/IL-17A ELISA Kits » Human IL-17 Quantikine ELISA Kit

Human IL-17 Quantikine ELISA Kit

 (95 Publications)
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Catalog# & Conjugate Size Price
HRP
S1700
HRP
PD1700
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Human IL-17 Quantikine ELISA Kit Summary

Background
The Quantikine Human IL-17 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IL-17 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-17 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-17 accurately. Results obtained using natural human IL-17 sh...owed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-17.
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Specificity
Natural and recombinant human IL-17
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
IL17A

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using D1700.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-17 Quantikine ELISA Kit

  • CTLA8
  • CTLA-8
  • CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
  • Cytotoxic T-lymphocyte-associated antigen 8
  • IL17
  • IL-17
  • IL17A
  • IL-17A
  • IL-17Acytotoxic T-lymphocyte-associated protein 8
  • IL-17CTLA-8
  • IL17interleukin-17A
  • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
  • interleukin 17A

Background

Human Interleukin 17 (IL-17), also known as IL-17A and CTLA-8, is a 15-20 kDa, variably glycosylated polypeptide that belongs to the IL-17 family of cytokines (1-5). Its alternate name, CTLA-8, originated from rodent studies where an activated hybridoma was created from the fusion of a mouse cytotoxic and a rat T cell lymphoma cell line. The molecule of interest in this study was assumed to have come from the mouse cytotoxic lymphocyte cell (thus the CTL designation), whereas, in fact, it was a rat lymphocyte molecule. Human IL-17/17A is synthesized as a 155 amino acid (aa) precursor that contains a 23 aa signal sequence and a 133 aa mature region that possesses a cysteine-knot fold (4-6). In both human and mouse, there is one conserved N-linked glycosylation site that likely contributes 5 kDa to its native molecular weight. IL-17A forms both a 32-38 kDa disulfide-linked homodimer, and a 40-45 kDa covalent heterodimer with IL-17F (7-9). Most secreted IL-17A is in the form of the IL-17A:F heterodimer, however, the IL-17A:A homodimer is the most bioactive of the two forms (8). Mature human IL-17A is 61%, 74%, and 99% aa identical to mouse, porcine, and chimpanzee IL-17A, respectively (10-12). Mammalian cells known to produce IL-17 are the CD4+ Th17 T cells, Paneth cells, GR1+CD11b+ myeloid suppressor cells, CD27- gamma δ T cells, CD1+NK1.1- iNKT cells, and CD3- CD4+ LTi-like cells (9, 13-17). 
A high affinity receptor for human IL-17 has been reported, and appears to be a heteromultimer of IL-17RA and IL-17RC, likely in a 2:1 ratio (1, 18). IL-17RA is a 130 kDa, type I transmembrane glycoprotein that bears no resemblance to members of the cytokine, TNF or immunoglobulin receptor superfamily (2, 10, 15). IL-17RC is also a type I transmembrane protein, approximately 90-95 kDa in size, that shares less than 30% aa identity with IL-17RA (19, 20). Both receptors are needed for IL-17A and IL-17A:F activity. The two receptors appear to form a functional association following ligand binding to IL-17RA (1, 21, 22). 
IL-17 is best known for its participation in the recruitment and survival of neutrophils (14, 15, 23, 24, 25). Its induction was initially described to be the result of antigen stimulation of dendritic cells, resulting in IL-23 secretion. In a T cell receptor-independent event, IL-23 induces T cell production of IL-17 (14). Once secreted, IL-17 in the bone marrow would seem to induce stromal/fibroblast expression of both G-CSF and stem cell factor (membrane form), an effect that increases polymorphonuclear neutrophils (PMN) differentiation and production. IL-17 may complement this by directly blocking neutrophil apoptosis, promoting greater circulating PMN numbers (23). In the tissues, IL-17 would also seem to promote neutrophil extravasation, principally through its effects on macrophages and endothelial cells (EC). On macrophages, IL-17 induces TNF-alpha , IL-1b and IL-6 production (26). TNF-alpha and IL-1b then act on local ECs to induce G-CSF secretion, an effect that is potentiated by IL-17 (27). IL-17 further contributes to PMN influx by inducing EC CXC chemokine release and NO production, which may increase vascular permeability (14, 28). IL-17 effects are not limited to inflammation. In synovial joints, IL-17 upregulates RANKL expression on osteoblasts. This provides a stimulus for osteoclast formation and subsequent bone resorption (24). In conjunction with IL-4 and CD40L, IL-17A also promotes the generation of IgE secreting cells (29). And in white fat, IL-17A inhibits adipocyte differentiation from preadipocytes, and impairs glucose uptake by mature adipocytes (30).

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Human IL-17
Quantikine ELISA Kit
D1700
Human IL-17 Quantikine ELISA Kit
Species: Hu
Applications: ELISA
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-17/IL-17A (D1700)(95)

We have publications tested in 2 confirmed species: Human, Mouse.


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Human
(95)
Mouse
(1)
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Showing Publications 1 - 10 of 95. Show All 95 Publications.
Publications using D1700 Applications Species
Karwaciak, I;Pastwi?ska, J;Sa?kowska, A;Bachorz, RA;Ratajewski, M; Evaluation of the Activity of Cardiac Glycosides on ROR? and ROR?T Nuclear Receptors Archives of biochemistry and biophysics 2024-07-04 [PMID: 38971421] (Human) Human
Kutuk, MO;Tufan, AE;Kilicaslan, F;Gokcen, C;Aksu, GG;Yektas, C;Kandemir, H;Celik, F;Mutluer, T;Buber, A;Karadag, M;Coban, N;Coskun, S;Hangul, Z;Altintas, E;Acikbas, U;Giray, A;Aka, Y;Basturk, B;Kutuk, O; Cytokine expression profiles in children and adolescents with tic disorders Scientific reports 2024-07-02 [PMID: 38956051] (Human) Human
Scovino, AM;Dahab, EC;Diniz-Lima, I;de Senna Silveira, E;Barroso, SPC;Cardoso, KM;Nico, D;Makhoul, GJ;da Silva-Junior, EB;Freire-de-Lima, CG;Freire-de-Lima, L;Fonseca, LMD;Valente, N;Nacife, V;Machado, A;Araújo, M;Vieira, GF;Pauvolid-Corrêa, A;Siqueira, M;Morrot, A; A Comparative Analysis of Innate Immune Responses and the Structural Characterization of Spike from SARS-CoV-2 Gamma Variants and Subvariants Microorganisms 2024-04-02 [PMID: 38674664] (Human) Human
CV Gonçalves, IS Ribeiro, MPL Galantini, IPR Muniz, PHB Lima, GS Santos, RAA da Silva Inflammaging and body composition: New insights in diabetic and hypertensive elderly men Experimental gerontology, 2022-10-28;170(0):112005. 2022-10-28 [PMID: 36341786] (Human) Human
J Milosevic, M Jurisevic, V Grbovic, I Jovanovic, N Gajovic, A Jurisic-Sk Potential Protective Role of Galectin-3 in Patients with Gonarthrosis and Diabetes Mellitus: A Cross-Sectional Study International Journal of Environmental Research and Public Health, 2022-09-13;19(18):. 2022-09-13 [PMID: 36141752] (Human) Human
Q Su, L Zhang, Z Qi, B Huang The Mechanism of Inflammatory Factors and Soluble Vascular Cell Adhesion Molecule-1 Regulated by Nuclear Transcription Factor NF-kappaB in Unstable Angina Pectoris Journal of Immunology Research, 2022-07-30;2022(0):6137219. 2022-07-30 [PMID: 35942210] (Human) Human
MPL Galantini, IS Ribeiro, CV Gonçalves, IPR Muniz, PHB Lima, GS Santos, RAA da Silva The sweet fuel of inflammation: New perspectives on the complex web that interconnects diabetes Experimental gerontology, 2022-07-30;167(0):111905. 2022-07-30 [PMID: 35918042] (Human) Human
S Luo, R Wu, Q Li, G Zhang Epigenetic Regulation of IFI44L Expression in Monocytes Affects the Functions of Monocyte-Derived Dendritic Cells in Systemic Lupus Erythematosus Journal of Immunology Research, 2022-05-10;2022(0):4053038. 2022-05-10 [PMID: 35592687] (Human) Human
U Förster-Ru, AJ Szczepek, G Pierchalla, JW Fluhr, M Artuc, T Zuberbier, C Bachert, H Olze Chemokine Expression-Based Endotype Clustering of Chronic Rhinosinusitis Journal of personalized medicine, 2022-04-18;12(4):. 2022-04-18 [PMID: 35455762] (Human) Human
J Pastwi?ska, K Kara?, A Sa?kowska, I Karwaciak, K Cha?a?kiew, BA Wojtczak, RA Bachorz, M Ratajewski Identification of Corosolic and Oleanolic Acids as Molecules Antagonizing the Human RORgammaT Nuclear Receptor Using the Calculated Fingerprints of the Molecular Similarity International Journal of Molecular Sciences, 2022-02-08;23(3):. 2022-02-08 [PMID: 35163824] (Human) Human
Show All 95 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-17/IL-17A (D1700). (Showing 1 - 2 of 2 FAQs).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
  2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
    • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

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Bioinformatics

Gene Symbol IL17A