![Western Blot: HspB11 Antibody [NBP2-94113] - Western blot analysis of extracts of various cell lines, using HspB11 Rabbit pAb (NBP2-94113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/HspB11 Antibody-Western Blot-NBP2-94113-img0004.jpg "Western Blot: HspB11 Antibody [NBP2-94113] - Western blot analysis of extracts of various cell lines, using HspB11 Rabbit pAb (NBP2-94113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human HSPB11 (NP_057210.2). MRKIDLCLSSEGSEVILATSSDEKHPPENIIDGNPETFWTTTGMFPQEFIICFHKHVRIERLVIQSYFVQTLKIEKSTSKEPVDFEQWIEKDLVHTEGQLQNEEIVAHDGSATYLRFIIVSAFDHFASVHSVSAEGTVVSNLSS |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | IFT25 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 16 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Affinity purified |
![Western Blot HSP10/EPF Antibody [Unconjugated]](https://images.novusbio.com/images2/HSP10_AF2584_Western_Blot_5198.jpg)
![Simple Western HSP10/EPF Antibody [Unconjugated]](https://images.novusbio.com/images2/HSP10_AF2584_Simple_Western_17132.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | IFT25 |
![Immunocytochemistry/Immunofluorescence: HspB11 Antibody [NBP2-94113] - Analysis of L929 cells using HspB11 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HspB11-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94113-img0001.jpg "Immunocytochemistry/Immunofluorescence: HspB11 Antibody [NBP2-94113] - Analysis of L929 cells using HspB11 at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: HspB11 Antibody [NBP2-94113] - Paraffin-embedded human placenta using HspB11 .](http://images.novusbio.com/fullsize/HspB11-Antibody-Immunohistochemistry-Paraffin-NBP2-94113-img0002.jpg "Immunohistochemistry-Paraffin: HspB11 Antibody [NBP2-94113] - Paraffin-embedded human placenta using HspB11 .")
![Immunohistochemistry-Paraffin: HspB11 Antibody [NBP2-94113] - Paraffin-embedded mouse lung using HspB11 .](http://images.novusbio.com/fullsize/HspB11-Antibody-Immunohistochemistry-Paraffin-NBP2-94113-img0003.jpg "Immunohistochemistry-Paraffin: HspB11 Antibody [NBP2-94113] - Paraffin-embedded mouse lung using HspB11 .")
![Western Blot HSP20/HSPB6 Antibody (494310) [Unconjugated]](https://images.novusbio.com/images2/HSP20_MAB4200_Western_Blot_6159.jpg)
![Simple Western HSP20/HSPB6 Antibody (494310) [Unconjugated]](https://images.novusbio.com/images/antibody/mab4200_human-mouse-rat-hsp20-hspb6-mab-clone-494310-simple-western-27112024131924..jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_587.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_588.jpg)
![N/A BDNF [HRP]](https://images.novusbio.com/images2/BDNF_DBD00_ELISA_36.jpg)
![Western Blot HSPB8/HSP22 Antibody (520817) [Unconjugated]](https://images.novusbio.com/images2/HSPB8_MAB4987_Western_Blot_9457.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")