![SDS-Page: Recombinant Human HSPA8/HSC71/Hsc70 Protein [NBP1-30278] - Analysis of Human protein.](http://images.novusbio.com/fullsize/Recombinant-Human-HSPA8-HSC71-Hsc70-Protein-SDS-Page-NBP1-30278-img0001.jpg "SDS-Page: Recombinant Human HSPA8/HSC71/Hsc70 Protein [NBP1-30278] - Analysis of Human protein.")
| Reactivity | HuSpecies Glossary |
| Applications | Func, PAGE |
| Concentration | 1 mg/ml |
| Description | A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-646 of Human HSPA8/HSC71/Hsc70 Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSKGPAVGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL IGDAAKNQVA MNPTNTVFDA KRLIGRRFDD AVVQSDMKHW PFMVVNDAGR PKVQVEYKGE TKSFYPEEVS SMVLTKMKEI AEAYLGKTVT NAVVTVPAYF NDSQRQATKD AGTIAGLNVL RIINEPTAAA IAYGLDKKVG AERNVLIFDL GGGTFDVSIL TIEDGIFEVK STAGDTHLGG EDFDNRMVNH FIAEFKRKHK KDISENKRAV RRLRTACERA KRTLSSSTQA SIEIDSLYEG IDFYTSITRA RFEELNADLF RGTLDPVEKA LRDAKLDKSQ IHDIVLVGGS TRIPKIQKLL QDFFNGKELN KSINPDEAVA YGAAVQAAIL SGDKSENVQD LLLLDVTPLS LGIETAGGVM TVLIKRNTTI PTKQTQTFTT YSDNQPGVLI QVYEGERAMT KDNNLLGKFE LTGIPPAPRG VPQIEVTFDI DANGILNVSA VDKSTGKENK ITITNDKGRL SKEDIERMVQ EAEKYKAEDE KQRDKVSSKN SLESYAFNMK ATVEDEKLQG KINDEDKQKI LDKCNEIINW LDKNQTAEKE EFEHQQKELE KVCNPIITKL YQSAGGMPGG MPGGFPGGGA PPSGGASSGP TIEEVD |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Gene | HSPA8 |
| Purity | >90%, by SDS-PAGE |
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| Application Notes | Use in In vitro reported in scientific literature (PMID: 33859739). |
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| Theoretical MW | 73.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | >90%, by SDS-PAGE |
Research Areas for HSPA8/HSC71/Hsc70 Recombinant Protein (NBP1-30278)Find related products by research area.
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| Gene Symbol | HSPA8 |
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![Immunocytochemistry/Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a HSP70 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](https://images.novusbio.com/images/Hsp70-Hsc70-Antibody-13D3-Immunocytochemistry-Immunofluorescence-NB120-2788-img0010.jpg "Immunocytochemistry/Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a HSP70 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.")