![Western Blot: Hsc70 Overexpression Lysate (Adult Normal) [NBL1-11755] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Hsc70.](http://images.novusbio.com/fullsize/Hsc70-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11755-img0002.jpg "Western Blot: Hsc70 Overexpression Lysate (Adult Normal) [NBL1-11755] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Hsc70.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Description | HSPA8/HSC71/Hsc70 Transient Overexpression LysateExpression Host: HEK293T Plasmid: RC202209 Accession#: NM_006597 Protein Tag: C-MYC/DDK You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT. |
| Gene | HSPA8 |
| Dilutions |
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| Application Notes | This product is intended for use as a positive control in Western Blot. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading. |
| Theoretical MW | 70.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | RIPA buffer |
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| Gene Symbol | HSPA8 |
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![Immunocytochemistry/Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a HSP70 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](https://images.novusbio.com/images/Hsp70-Hsc70-Antibody-13D3-Immunocytochemistry-Immunofluorescence-NB120-2788-img0010.jpg "Immunocytochemistry/Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a HSP70 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.")