![Western Blot: HSP27 Antibody [NB120-5579] - Analysis of 50ug of the indicated whole cell lysates per well.](http://images.novusbio.com/fullsize/HSP27-Antibody-Western-Blot-NB120-5579-img0003.jpg "Western Blot: HSP27 Antibody [NB120-5579] - Analysis of 50ug of the indicated whole cell lysates per well.")
| Reactivity | Hu, Rt, Pm, CaSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Synthetic peptide corresponding to residues L(10) L R G P S W D P F R C(21) of human Heat Shock Protein 27. |
| Localization | Hsp27 is cytoplasmic in interphase cells, localising to the nucleus under heat shock conditions. Hsp27 localises to mitotic spindle microtubules. |
| Specificity | Hsp27 |
| Predicted Species | Canine (90%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HSPB1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | WB: Detects an approx. 27 kDa which corresponds to hsp27 from HeLa cells.ICC: Staining of hsp27 in HeLa cells with this antibody results in diffuse cytoplasmic staining before heat shock and diffuse cytoplasmic along with punctate staining patterns after. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 1 mg/ml BSA |
| Preservative | 0.05% Sodium Azide |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||
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reviewed by:
Liyong Zhang |
WB | Human | 08/16/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in MCF-7 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5579-img0007.jpg "Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in MCF-7 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.")
![Immunohistochemistry-Paraffin: HSP27 Antibody [NB120-5579] - Both normal and cancer biopsies of deparaffinized human Breast carcinoma tissue.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunohistochemistry-Paraffin-NB120-5579-img0009.jpg "Immunohistochemistry-Paraffin: HSP27 Antibody [NB120-5579] - Both normal and cancer biopsies of deparaffinized human Breast carcinoma tissue.")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with HSP27 polyclonal antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5579-img0004.jpg "Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with HSP27 polyclonal antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in C6 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5579-img0005.jpg "Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in C6 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5579-img0006.jpg "Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.")
![Immunohistochemistry-Paraffin: HSP27 Antibody [NB120-5579] - Both normal and cancer biopsies of deparaffinized human Skeletal muscle tissue.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunohistochemistry-Paraffin-NB120-5579-img0008.jpg "Immunohistochemistry-Paraffin: HSP27 Antibody [NB120-5579] - Both normal and cancer biopsies of deparaffinized human Skeletal muscle tissue.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
-(01-mg)_NB120-5579_9401.jpg)