Human HSF1 ELISA Kit (Colorimetric) Summary
Description |
This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples. |
Standard Curve Range |
0.625 - 40 ng/mL (example only; lot dependent) |
Sensitivity |
0.5 ng/ml (example only; lot dependent) |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 9.80% (example only; lot dependent) |
Intra-Assay |
CV% < 4.90% (example only; lot dependent) |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
HSF1 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage is content dependent. |
Alternate Names for Human HSF1 ELISA Kit (Colorimetric)
Background
Human cells respond to heat stress by inducing the binding of a pre-existing transcriptional activator (heat shock factor, HSF) to DNA (1). Induction of heat shock protein (HSP) gene expression by stress is initiated by binding of HSF1 to HSP gene promoters to increase their transcription. The cytoprotective functions of these HSPs are essential for cell survival, and thus it is critical that inducible HSP gene expression be executed rapidly and efficiently. There is an interaction between heat shock factor 1 (HSF1) and symplekin, a protein known to form a complex with the polyadenylation factors CstF and CPSF. HSF1-symplekin complexes are detected only after stress treatment, and these two proteins co-localize in punctate nuclear structures in stressed cells (2). A chaperone/Hsp functioning as repressor of heat shock transcription factor (HSF) could make activation of hsp genes dependent on protein unfolding. It has been concluded that Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of HSF1 (3).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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