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Hormone-sensitive Lipase/HSL Antibody (729923)

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Western blot shows lysates of MCF-7 human breast cancer cell line, NTera-2 human testicular embryonic carcinoma cell line, SVEC4-10 mouse vascular endothelial cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Hormone-sensitive Lipase/HSL Monoclonal Antibody (Catalog # MAB7104) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Hormone-sensitive Lipase/HSL at approximately 70 kDa and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of MCF-7 human breast cancer cell line, NTera-2 human testicular embryonic carcinoma cell line, SVEC4-10 mouse vascular endothelial cell line, and HT-29 human colon adenocarcinoma cell line. ...read more
Hormone-sensitive Lipase/HSL was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human Hormone-sensitive Lipase/HSL Monoclonal Antibody (Catalog # MAB7104) at 8 µg/mL overnight ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
729923
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7104-SP
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Hormone-sensitive Lipase/HSL Antibody (729923) Summary

Immunogen
E. coli-derived recombinant human Hormone-sensitive Lipase/HSL
Met302-Leu425
Accession # Q05469
Specificity
Detects human Hormone-sensitive Lipase/HSL in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
LIPE
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publication using MAB7104.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Hormone-sensitive Lipase/HSL Antibody (729923)

  • hormone-sensitive lipase testicular isoform
  • Hormonesensitive Lipase
  • Hormone-sensitive Lipase
  • HSL
  • HSLEC 3.1.1.79
  • LHS
  • lipase, hormone-sensitive
  • LIPE

Background

HSL (hormone-sensitive lipase), also called LIPE, is an 84 kDa phosphoprotein that is the rate-limiting enzyme in the catalytic breakdown of triglycerides. The human 775 amino acid (aa) form is active in adipose tissue and skeletal muscle. Within the region used as an immunogen, human HSL shares 92% and 93% aa sequence identity with mouse and rat HSL, respectively. HSL translocates to triglyceride-metabolizing lipid droplets in response to epinephrine or contraction in skeletal muscle, but to the cytosol following insulin treatment of adipocytes. A 120 kDa isoform of human HSL with a 301 aa N-terminal extension is present in the testes, localized to elongating spermatids and spermatozoa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Hormone-sensitive Lipase/HSL Antibody (MAB7104)(1)


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Bioinformatics

Gene Symbol LIPE
Uniprot