![Western Blot: HMGCS1 Antibody [NBP2-16861] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HMGCS1 antibody (NBP2-16861) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16861_rabbit-polyclonal-hmgcs1-antibody-910202420134033.jpg "Western Blot: HMGCS1 Antibody [NBP2-16861] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HMGCS1 antibody (NBP2-16861) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HMGCS1. The exact sequence is proprietary. |
| Localization | Cytoplasm |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HMGCS1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 57 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for HMGCS1 Antibody (NBP2-16861)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: HMGCS1 Antibody [NBP2-16861] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGCS1 protein stained by HMGCS1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/HMGCS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16861-img0006.jpg "Immunocytochemistry/Immunofluorescence: HMGCS1 Antibody [NBP2-16861] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGCS1 protein stained by HMGCS1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: HMGCS1 Antibody [NBP2-16861] - Mouse liver. HMGCS1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HMGCS1-Antibody-Immunohistochemistry-Paraffin-NBP2-16861-img0007.jpg "Immunohistochemistry-Paraffin: HMGCS1 Antibody [NBP2-16861] - Mouse liver. HMGCS1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: HMGCS1 Antibody [NBP2-16861] - Mouse intestine. HMGCS1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HMGCS1-Antibody-Immunohistochemistry-Paraffin-NBP2-16861-img0005.jpg "Immunohistochemistry-Paraffin: HMGCS1 Antibody [NBP2-16861] - Mouse intestine. HMGCS1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: HMGCS1 Antibody [NBP2-16861] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HMGCS1 antibody (NBP2-16861) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16861_rabbit-polyclonal-hmgcs1-antibody-91020242001023.jpg "Western Blot: HMGCS1 Antibody [NBP2-16861] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HMGCS1 antibody (NBP2-16861) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")