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Mouse HMGB1/HMG-1 ELISA Kit (Chemiluminescence)

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 (1 Review)
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ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Chemiluminescence) [NBP2-62782] - Samples were spiked with high concentrations of Mouse HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Chemiluminescence) [NBP2-62782] - Samples were spiked with high concentrations of Mouse HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Chemiluminescence) [NBP2-62782] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
7.81 - 500 pg/mL
Sensitivity
4.69 pg/mL

Order Details

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Mouse HMGB1/HMG-1 ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Mouse HMGB-1 in samples. No significant cross-reactivity or interference between Mouse HMGB-1 and analogues was observed.
Standard Curve Range
7.81 - 500 pg/mL
Sensitivity
4.69 pg/mL
Assay Type
Sandwich-CLIA
Inter-Assay
%CV < 8
Intra-Assay
%CV < 8
Spike Recovery
90 - 110%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
HMGB1

Applications/Dilutions

Dilutions
  • ELISA
Reviewed Applications
Read 1 Review rated 4
using
NBP2-62782 in the following application:
Publications
Read Publications using
NBP2-62782 in the following applications:

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro CLIA Plate
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Substrate Reagent A
  12. Substrate Reagent B

Alternate Names for Mouse HMGB1/HMG-1 ELISA Kit (Chemiluminescence)

  • Amphoterin
  • high mobility group box 1
  • High mobility group protein 1
  • high mobility group protein B1
  • high-mobility group (nonhistone chromosomal) protein 1
  • high-mobility group box 1
  • HMG1
  • HMG-1
  • HMG1DKFZp686A04236
  • HMG3
  • HMGB1
  • SBP-1
  • Sulfoglucuronyl carbohydrate binding protein

Background

HMGB1 and HMGB2 are part of the chromatin non-histone high mobility group proteins 1 and 2. HMGB1 and HMGB2 (containing multiple HMG-boxes) are conserved domains of 80 amino acids which mediate the DNA binding of many proteins. HMG box domains recognize DNA structure. Both HMGB1 and HMGB2 contain a N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of HMGB1 and HMGB2 contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMGB1 and HMGB2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites. HMGB1 and HMGB2 may also serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription, and replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for HMGB1/HMG-1 ELISA Kit (NBP2-62782)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ELISA.


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ELISA
(2)
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Mouse
(1)
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Review for HMGB1/HMG-1 ELISA Kit (NBP2-62782) (1) 41

Average Rating: 4
(Based on 1 review)

Reviews using NBP2-62782:
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HMGB1/HMG-1 NBP2-62782
Enlarge 		4
reviewed by:
Steven Moore
 10/13/2020
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Summary

Sample TestedELISA Kit,Mouse HMGB1/HMG-1 ELISA Kit
LotMouse HMGB1/HMG-1

Comments

CommentsMouse Kit

Product General Protocols

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FAQs for HMGB1/HMG-1 ELISA Kit (NBP2-62782) (0)

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Contact Information

Product PDFs

  SDS
  Mouse HMGB1/HMG-1 ELISA Kit (Chemiluminescence) Manual
  PDF Datasheet
  Request CofA

Recent Reviews

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HMGB1/HMG-1 NBP2-62782
Enlarge
 Steven Moore
10/13/2020
Application:
Species:
read this review

Bioinformatics

Gene Symbol HMGB1