HLA G Antibody (87G) - BSA Free

Human leukocyte antigen (HLA)-G is a non-classical major histocompatibility complex (MHC) class I gene belonging to the HLA class I family. The HLA class I family is subdivided into the classical (HLA-Ia) antigens comprised of HLA-A, -B, and -C molecules and the nonclassical (HLA-Ib) group including the HLA-E, -F, and -G molecules. Seven different splice variants of HLA-G have been observed including four membrane isoforms (HLA-G1, HLA-G2, HLA-G3, and HLA-G4) and three soluble isoforms (HLA-G5, HLA-G6 and HLA-G7). Proteolytic processing of HLA-G1 by metalloproteinases (MMPs) such as MMP-2 produces the soluble isoform, shedding HLA-G1. The HLA-G1 and HLA-G5 isoforms share a similar confirmation to the classical MHC class I protein, consisting of three extracellular domains including alpha1, alpha2, and alpha3 associated with Beta-2-microglobulin (B2M). Other HLA-G variants are shorter, missing one or two of the globular domains and are not bound to B2M. HLA-G receptors include KIR2DL4 (CD158d), with the highest affinity for ILT2 (LILRB1, CD85j) and ILT4 (LILRB2, CD85d) (1,2).

Discovered in cytotrophoblasts, HLA-G is involved in fetal maternal immune tolerance and some studies have linked its downregulation with severe preeclampsia (3). HLA-G mediated immune suppression works by impeding cell proliferation, differentiation, cytotoxicity, cytokine secretion and chemotaxis; and activation of regulatory cells and MDSCs or M2 type macrophage. HLA-G is constitutively expressed in immune-privileged sites such as pancreatic islets, mesenchymal stem cells (MSCs) and the cornea. Upregulation of HLA-G occurs in pathological states including cancer, allo-transplantations, viral infections, autoimmune and inflammatory diseases (4). In cancer, HLA-G expression is induced by hypoxia and has been correlated with advanced tumor stage, tumor metastasis, and poor therapeutic response and survival. HLA-G is an attractive tumor associated-antigen (TAA) for immunotherapy and is considered a major immune checkpoint (ICP).

References

1. Loustau, M., Anna, F., Drean, R., Lecomte, M., Langlade-Demoyen, P., & Caumartin, J. (2020). HLA-G Neo-Expression on Tumors. Front Immunol, 11:1685. PMID: 32922387

2. Lin A, Yan WH. (2018) Heterogeneity of HLA-G Expression in Cancers: Facing the Challenges. Front Immunol. 9:2164. PMID: 30319626

3. Djurisic S, Hviid TV. (2014) HLA Class Ib Molecules and Immune Cells in Pregnancy and Preeclampsia. Front Immunol. 5:652. PMID: 25566263

4. Lila N, Rouas-Freiss N, Dausset J, Carpentier A, Carosella ED. (2001) Soluble HLA-G protein secreted by allo-specific CD4+ T cells suppresses the allo-proliferative response: a CD4+ T cell regulatory mechanism. Proc Natl Acad Sci U S A. 98(21):12150-12155. PMID: 11572934

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 7 applications: B/N, ELISA, FLOW, ICC/IF, IF/IHC, IHC-Fr, WB.

Showing Publications 1 - 10 of 13. Show All 13 Publications. Collapse Publications.

Publications using NBP1-43123	Applications	Species
Levy S, Somasundaram L, Raj IX et al. dCas9 fusion to computer-designed PRC2 inhibitor reveals functional TATA box in distal promoter region Cell reports 2022-03-01 [PMID: 35235780] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Mestrallet G, Carosella ED, Martin MT et al. Immunosuppressive Properties of Epidermal Keratinocytes Differ According to Their Immaturity Status Frontiers in immunology 2022-02-11 [PMID: 35222373] (FLOW, Human)	FLOW	Human
Mestrallet G, AuvrE F, Schenowitz C et al. Human Keratinocytes Inhibit CD4+ T-Cell Proliferation through TGFB1 Secretion and Surface Expression of HLA-G1 and PD-L1 Immune Checkpoints Cells 2021-06-08 [PMID: 34201301] (IF/IHC, FLOW, Human)	IF/IHC, FLOW	Human
Dissanayake K, NOmm M, LAttekivi F et al. Individually cultured bovine embryos produce extracellular vesicles that have the potential to be used as non-invasive embryo quality markers Theriogenology 2020-06-01 [PMID: 32259747] (Human)		Human
Lin A, Yan WH, Xu HH et al. HLA-G expression in human ovarian carcinoma counteracts NK cell function. Ann Oncol. 2007-11-01 [PMID: 17846022] (B/N, FLOW, Human)	B/N, FLOW	Human
Ishitani A, Sageshima N, Lee N et al. Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition. J Immunol. 2003-08-01 [PMID: 12874228] (IF/IHC, Human)	IF/IHC	Human
Contini P, Ghio M, Poggi A et al. Soluble HLA-A,-B,-C and -G molecules induce apoptosis in T and NK CD8+ cells and inhibit cytotoxic T cell activity through CD8 ligation. Eur J Immunol. 2003-01-01 [PMID: 12594841] (B/N, ELISA, Human)	B/N, ELISA	Human
Hurks H, Valter M, Wilson L et al. Uveal melanoma: no expression of HLA-G. Invest Ophthalmol Vis Sc 2001 Dec [PMID: 11726606] (IHC-Fr )	IHC-Fr
Hurks H, Valter M, Wilson L et al. Uveal melanoma: no expression of HLA-G. Invest Ophthalmol Vis Sci. 2001 Dec [PMID: 11726606] (IHC-Fr )	IHC-Fr
Lila N, Rouas-Freiss N, Dausset J et al. Soluble HLA-G protein secreted by allo-specific CD4+ T cells suppresses the allo-proliferative response: a CD4+ T cell regulatory mechanism. Proc Natl Acad Sci U S A. 2001-10-09 [PMID: 11572934] (ICC/IF, FLOW, Human)	ICC/IF, FLOW	Human
Le Gal FA, Riteau B, Sedlik C et al. HLA-G-mediated inhibition of antigen-specific cytotoxic T lymphocytes. Int Immunol. 1999-08-01 [PMID: 10421792] (FLOW, Human)	FLOW	Human
Moreau P, Adrian-Cabestre F, Menier C et al. IL-10 selectively induces HLA-G expression in human trophoblasts and monocytes. Int Immunol. 1999-05-01 [PMID: 10330285] (FLOW, ICC/IF, Human)	FLOW, ICC/IF	Human
Paul P, Cabestre F A et al. Heterogeneity of HLA-G gene transcription and protein expression in malignant melanoma biopsies. Cancer Res 1999-04-15 [PMID: 10213506] (ICC/IF, Human)	ICC/IF	Human
Show All 13 Publications. Collapse Publications.

Array NBP1-43123

• HLA G Antibodies
• HLA G ELISA Kit
• HLA G Lysate
• HLA G Protein