![Immunocytochemistry/Immunofluorescence: Histone H3.1 Antibody [NB100-81964] - Staining of methanol-fixed Hela cells using NB100-81964](http://images.novusbio.com/fullsize/antibody/Histone-H3.jpg "Immunocytochemistry/Immunofluorescence: Histone H3.1 Antibody [NB100-81964] - Staining of methanol-fixed Hela cells using NB100-81964")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The antiserum was produced against synthesized non-phosphopeptide derived from human Histone H3.1 around amino acids 8~12 (R-K-S-T-G). |
| Specificity | Detects endogenous levels of total Histone H3.1 protein. NB100-81964 is also cross-reactive with Histone H3.2 and H3.3 from human mouse and rat. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | H3C6 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Theoretical MW | 17 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Western Blot Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7)](https://images.novusbio.com/images/nbp3-33534_rabbit-histone-h2ae-hydroxy-tyr39-mab-2j2q7-1012202411343397.jpg)
![Western Blot Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7)](https://images.novusbio.com/images/nbp3-33534_rabbit-histone-h2ae-hydroxy-tyr39-mab-2j2q7-1012202411404011.jpg)
![N/A Histone H2AE [Hydroxy Tyr39] Antibody (2J2Q7)](https://images.novusbio.com/images/nbp3-33534_rabbit-histone-h2ae-hydroxy-tyr39-mab-2j2q7-31120251659369.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Histone H3.1 Antibody (NB100-81964)Find related products by research area.
|
|
H3.1 - A core histone essential for genome storage and organization Histones are the main protein component of chromatin and are essential for the storage and compaction of the genome. DNA wraps around histone oligomers to make up nucleosomes, the individual subunits of chromatin. By altering the accessibility of t... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: Histone H3. 1 Antibody [NB100-81964] - Western blot analysis of extracts of various cell lines, using NB100-81964](http://images.novusbio.com/fullsize/nb100-81964_rabbit-polyclonal-histone-h3-1-antibody-23420241452105.jpg "Western Blot: Histone H3. 1 Antibody [NB100-81964] - Western blot analysis of extracts of various cell lines, using NB100-81964")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")