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HEY1 Recombinant Protein Antigen

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Summary
Reactivity HuSpecies Glossary
Applications AC

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HEY1 Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human HEY1.

Source: E. coli

Amino Acid Sequence: MKRAHPEYSSSDSELDETIEVEKESADENGNLSSALGSMSPTTSSQILARKR

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.
Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
HEY1
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-56068.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for HEY1 Recombinant Protein Antigen

  • basic helix-loop-helix protein OAF1
  • BHLHb31
  • Cardiovascular helix-loop-helix factor 2
  • CHF-2
  • CHF2hHRT1
  • Class B basic helix-loop-helix protein 31
  • Hairy and enhancer of split-related protein 1
  • hairy/enhancer-of-split related with YRPW motif 1
  • hairy/enhancer-of-split related with YRPW motif protein 1
  • Hairy-related transcription factor 1
  • HERP2HES-related repressor protein 1
  • HESR-1
  • HESR1MGC1274
  • HES-related repressor protein 2
  • HRT1
  • HRT-1OAF1

Background

Hairy/enhancer of split-related proteins, such as HEY1, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. Hey1 functions as a corepressor, providing a mechanism of cross-talk between Notch and androgen signaling pathways. It is excluded from the nucleus in most human prostate cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol HEY1