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![Western Blot: HEY1 Antibody [NBP2-16818] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HEY1-Antibody-Western-Blot-NBP2-16818-img0004.jpg "Western Blot: HEY1 Antibody [NBP2-16818] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: HEY1 Antibody [NBP2-16818] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HEY1 protein stained by HEY1 antibody diluted at 1:2000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HEY1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16818-img0002.jpg)
![Immunohistochemistry-Frozen: HEY1 Antibody [NBP2-16818] - Hey1 staining on E11.5 mouse Pharyngeal Mesoderm. Fixed 4% PFA overnight. Blocked with 1% BSA. Primary antibody dilution at 1:100. Secondary antibody at 1:1000, conjugated to Alexa Fluor 488. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HEY1-Antibody-Immunohistochemistry-Frozen-NBP2-16818-img0005.jpg)
![Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (NBP3-25502) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25502_rabbit-hey1-mab-hl2367-azide-and-bsa-free-2120249351339.jpg "Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (NBP3-25502) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] - Whole zebrafish extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (NBP3-25502) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25502_rabbit-hey1-mab-hl2367-azide-and-bsa-free-2120249362767.jpg)
![Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (NBP3-25502) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25502_rabbit-hey1-mab-hl2367-azide-and-bsa-free-2120249422655.jpg)
![Immunohistochemistry-Paraffin: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] - HEY1 antibody [HL2367] detects HEY1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. HEY1 stained by HEY1 antibody [HL2367] (NBP3-25502) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25502_rabbit-hey1-mab-hl2367-azide-and-bsa-free-212024943557.jpg)
![Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (NBP3-25502) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25502_rabbit-hey1-mab-hl2367-azide-and-bsa-free-2120249371561.jpg)
![Western Blot: HEY1 Antibody (HL2367) - Azide and BSA Free [NBP3-25502] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (NBP3-25502) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25502_rabbit-hey1-mab-hl2367-azide-and-bsa-free-212024943284.jpg)
![Western Blot: HEY1 Antibody [NBP2-27152] - Analysis of Hey1 in mouse embryo brain lysate using 1) preimmune sera at 1:5000 dilution and 2) purified NBP2-27152. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HEY1-Antibody-Western-Blot-NBP2-27152-img0002.jpg "Western Blot: HEY1 Antibody [NBP2-27152] - Analysis of Hey1 in mouse embryo brain lysate using 1) preimmune sera at 1:5000 dilution and 2) purified NBP2-27152. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: HEY1 Antibody (3B3) [H00023462-M01] - analysis of HEY1 expression in transfected 293T cell line by HEY1 monoclonal antibody (M01), clone 3B3. Lane 1: HEY1 transfected lysate (32.613 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HEY1-Antibody-3B3-Western-Blot-H00023462-M01-img0001.jpg "Western Blot: HEY1 Antibody (3B3) [H00023462-M01] - analysis of HEY1 expression in transfected 293T cell line by HEY1 monoclonal antibody (M01), clone 3B3. Lane 1: HEY1 transfected lysate (32.613 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: HEY1 Antibody (3B3) [H00023462-M01] - Analysis of monoclonal antibody to HEY1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/HEY1-Antibody-3B3-Immunocytochemistry-Immunofluorescence-H00023462-M01-img0004.jpg)
![ELISA: HEY1 Antibody (3B3) [H00023462-M01] - Detection limit for recombinant GST tagged HEY1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HEY1-Antibody-3B3-Sandwich-ELISA-H00023462-M01-img0005.jpg)
![Western Blot: HEY1 Antibody [NBP2-47436] - Analysis in control (vector only transfected HEK293T lysate) and HEY1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/HEY1-Antibody-Western-Blot-NBP2-47436-img0003.jpg "Western Blot: HEY1 Antibody [NBP2-47436] - Analysis in control (vector only transfected HEK293T lysate) and HEY1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: HEY1 Antibody [NBP2-47436] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/HEY1-Antibody-Immunohistochemistry-Paraffin-NBP2-47436-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: HEY1 Antibody [NBP3-35645] - Immunofluorescence analysis of A431 cells using HEY1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35645_rabbit-hey1-pab-1012202411531090.jpg "Immunocytochemistry/ Immunofluorescence: HEY1 Antibody [NBP3-35645] - Immunofluorescence analysis of A431 cells using HEY1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: HEY1 Antibody [NBP3-35645] - Western blot analysis of various lysates using HEY1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35645_rabbit-hey1-pab-101220241233830.jpg)
![Immunocytochemistry/Immunofluorescence: HEY1 Antibody [NBP2-56068] - Staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/HEY1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56068-img0001.jpg "Immunocytochemistry/Immunofluorescence: HEY1 Antibody [NBP2-56068] - Staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: HEY1 Antibody [NBP3-10943] - Immunohistochemical analysis of paraffin-embedded human lung tissue.](http://images.novusbio.com/fullsize/HEY1-Antibody-Immunohistochemistry-Paraffin-NBP3-10943-img0001.jpg "Immunohistochemistry-Paraffin: HEY1 Antibody [NBP3-10943] - Immunohistochemical analysis of paraffin-embedded human lung tissue.")
![SDS-Page: Recombinant Human HEY1 Protein [H00023462-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00023462-P01-1.jpg "SDS-Page: Recombinant Human HEY1 Protein [H00023462-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: HEY1 Antibody [NBP1-82377] - Rat Muscle Lysate 1.0 ug/ml, gel concentration 12%](http://images.novusbio.com/fullsize/HEY1-Antibody-Western-Blot-NBP1-82377-img0001.jpg "Western Blot: HEY1 Antibody [NBP1-82377] - Rat Muscle Lysate 1.0 ug/ml, gel concentration 12%")
![Western Blot: HEY1 Overexpression Lysate (Adult Normal) [NBL1-11520] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HEY1.](http://images.novusbio.com/fullsize/HEY1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11520-img0002.jpg "Western Blot: HEY1 Overexpression Lysate (Adult Normal) [NBL1-11520] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HEY1.")
![Western Blot: HEY1 Overexpression Lysate (Adult Normal) [NBL1-11521] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HEY1.](http://images.novusbio.com/fullsize/HEY1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11521-img0002.jpg "Western Blot: HEY1 Overexpression Lysate (Adult Normal) [NBL1-11521] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HEY1.")