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Hemoglobin zeta Antibody (778633)

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Western blot shows lysates of TF-1 human erythroleukemic cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Hemoglobin zeta Monoclonal Antibody ...read more
Hemoglobin zeta was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human Hemoglobin zeta Monoclonal Antibody (Catalog # MAB7708) at 10 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
778633
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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Hemoglobin zeta Antibody (778633) Summary

Immunogen
E. coli-derived recombinant human Hemoglobin zeta
Met1-Arg142
Accession # P02008
Specificity
Detects human Hemoglobin zeta in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
HBZ
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Hemoglobin zeta Antibody (778633)

  • HBAZ
  • HBZ
  • HBZ2
  • hemoglobin subunit zeta
  • Hemoglobin zeta chain
  • Hemoglobin zeta
  • hemoglobin, zeta
  • zeta-globin

Background

Hemoglobin is a tetrameric heme-containing protein that is responsible for the transport of oxygen by red blood cells in the circulation. For most of fetal development and adulthood, hemoglobin consists of two alpha chains and two beta chains. Hemoglobin zeta (HBZ) is an approximately 15 kDa alpha chain-like protein that is produced during the first few weeks of embryogenesis until the onset of alpha chain expression. Its expression is prolonged in a0-thalassemia which is characterized by deficient alpha chain production. The Gower-1, Portland-1, and Portland-2 forms of hemoglobin consist of two zeta chains in association with either two epsilon, gamma, or beta chains, respectively. Human Hemoglobin zeta shares 79% amino acid sequence identity with mouse and rat Hemoglobin zeta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HBZ
Uniprot