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Human Hematopoietic Prostaglandin D Synthase/HPGDS ELISA Kit (Colorimetric)

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ELISA: Human Hematopoietic Prostaglandin D Synthase/HPGDS ELISA Kit (Colorimetric) [NBP3-38991] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
78.125 - 5000 pg/mL (example only; lot dependent)
Sensitivity
36 pg/mL (example only; lot dependent)

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Human Hematopoietic Prostaglandin D Synthase/HPGDS ELISA Kit (Colorimetric) Summary

Description
Assay Length: 4.5 hours
Standard Curve Range
78.125 - 5000 pg/mL (example only; lot dependent)
Sensitivity
36 pg/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
HPGDS

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Reagent A
  2. Detection Reagent B
  3. Diluent Buffer
  4. Instruction manual
  5. Plate sealer for 96 wells
  6. Pre-coated 96T strip plate
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human Hematopoietic Prostaglandin D Synthase/HPGDS ELISA Kit (Colorimetric)

  • EC 2.5.1.18
  • EC 5.3.99.2
  • glutathione S-transferase sigma
  • Glutathione S-transferase
  • Glutathione-dependent PGD synthase
  • Glutathione-requiring prostaglandin D synthase
  • GST class-sigma
  • GSTS
  • GSTShematopoietic prostaglandin D2 synthase
  • hematopoietic prostaglandin D synthase
  • HPGDS
  • H-PGDS
  • PGDS
  • PGDSglutathione-dependent PGD synthetase
  • Prostaglandin-H2 D-isomerase
  • PTGDS2

Background

Prostaglandin D synthase catalyzes the isomerization of PGH2 to produce PGD2. Prostaglandin D synthase induces sleep, regulates nociception, inhibits platelet aggregation, and acts as an allergic mediator. Two distinct types of PGD synthase have been identified, namely the lipocalin type enzyme (b-trace) and the hematopoietic enzyme. Lipocalin type prostaglandin D synthase is localized in the central nervous system and male genital organs of various mammals and the human heart. This enzyme has been identified as b-trace, which is a major protein in human cerebrospinal fluid. Hematopoietic prostaglandin D synthase is widely distributed in the peripheral tissues and is localized in the antigen-presenting cells, mast cells, and megakaryocytes. This enzyme, which requires glutathione for activity, belongs to the sigma-class of glutathione-S-transferases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol HPGDS