![Western Blot: Helios Antibody [NBP2-04050] - Detection of human Helios by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Helios antibody NBP2-04050 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. Helios was also immunoprecipitated by another rabbit anti-Helios antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/Helios-Antibody-Western-Blot-NBP2-04050-img0005.jpg "Western Blot: Helios Antibody [NBP2-04050] - Detection of human Helios by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Helios antibody NBP2-04050 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. Helios was also immunoprecipitated by another rabbit anti-Helios antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 25 and 75 of human IKAROS Family Zinc Finger 2 (Helios) using the numbering given in entry NP_057344.2 (GeneID 22807). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | IKZF2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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