![Western Blot: ZNFN1A4 Antibody [NBP3-12160] - Staining of Jurkat nuclear lysate (A) + Peptide (B) with antibody at 2 ug/mL, and staining of K562 nuclear lysate (C) + Peptide (D) with antibody at 0.1 ug/mL, (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ZNFN1A4-Antibody-Western-Blot-NBP3-12160-img0002.jpg "Western Blot: ZNFN1A4 Antibody [NBP3-12160] - Staining of Jurkat nuclear lysate (A) + Peptide (B) with antibody at 2 ug/mL, and staining of K562 nuclear lysate (C) + Peptide (D) with antibody at 0.1 ug/mL, (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Immunogen | Peptide with sequence C-RPTFIDRLANSLTKR corresponding to internal region according to NP_071910.3. |
| Predicted Species | Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | IKZF4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at -20C in the dark. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | IKZF4 |
![Immunocytochemistry/Immunofluorescence: ZNFN1A4 Antibody [NBP3-12160] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/ZNFN1A4-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12160-img0004.jpg "Immunocytochemistry/Immunofluorescence: ZNFN1A4 Antibody [NBP3-12160] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Flow Cytometry: ZNFN1A4 Antibody [NBP3-12160] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/ZNFN1A4-Antibody-Flow-Cytometry-NBP3-12160-img0005.jpg "Flow Cytometry: ZNFN1A4 Antibody [NBP3-12160] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: ZNFN1A4 Antibody [NBP3-12160] - Staining of Mouse Heart lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ZNFN1A4-Antibody-Western-Blot-NBP3-12160-img0001.jpg "Western Blot: ZNFN1A4 Antibody [NBP3-12160] - Staining of Mouse Heart lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: ZNFN1A4 Antibody [NBP3-12160] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/ZNFN1A4-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12160-img0003.jpg "Immunocytochemistry/Immunofluorescence: ZNFN1A4 Antibody [NBP3-12160] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
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