| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB73091-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF73091-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB73092-SP | |||
| Carrier Free | IC73092N-100UG | |||
| Carrier Free | IC73092G-100UG | |||
| Carrier Free | IC73092V-100UG | |||
| Carrier Free | IC73092U-100UG | |||
| Carrier Free | IC73092T-100UG | |||
| Carrier Free | IC73092S-100UG | |||
| Carrier Free | IC73092R-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37723 | |||
![Western Blot: Helios Antibody [NBP2-16802] - Jurkat whole cell extracts and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HELIOS antibody diluted by 1:500 and developed with Trident Sharp-ECL.](http://images.novusbio.com/fullsize/Helios-Antibody-Western-Blot-NBP2-16802-img0003.jpg "Western Blot: Helios Antibody [NBP2-16802] - Jurkat whole cell extracts and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HELIOS antibody diluted by 1:500 and developed with Trident Sharp-ECL.")
![Immunohistochemistry-Paraffin: Helios Antibody [NBP2-16802] - Human ovarian cancer, using HELIOS antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/HELIOS-Antibody-Immunohistochemistry-Paraffin-NBP2-16802-img0002.jpg)
![Western Blot: Helios Antibody [NBP2-04050] - Detection of human Helios by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Helios antibody NBP2-04050 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. Helios was also immunoprecipitated by another rabbit anti-Helios antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/Helios-Antibody-Western-Blot-NBP2-04050-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Helios Antibody [NBP2-04050] - Detection of human Helios by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Helios antibody NBP2-04050 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. Helios was also immunoprecipitated by another rabbit anti-Helios antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Helios Antibody [NBP2-04050] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Helios antibody NBP1-97741 used for IP at 6ug/mg lysate. Helios was also immunoprecipitated by rabbit anti-Helios antibody NBP2-04050, which recognizes an upstream epitope. For blotting immunoprecipitated Helios, NBP2-04050 was used at 0.4ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds. nds.](http://images.novusbio.com/fullsize/Helios-Antibody-Immunoprecipitation-NBP2-04050-img0004.jpg)
![Flow Cytometry: Helios Antibody (22F6) [NBP2-37723] - Staining of CD4+ human peripheral blood lymphocytes using anti-Helios (clone 22F6) PE-CyTM7.](http://images.novusbio.com/fullsize/Helios-Antibody-22F6-Flow-Cytometry-NBP2-37723-img0003.jpg "Flow Cytometry: Helios Antibody (22F6) [NBP2-37723] - Staining of CD4+ human peripheral blood lymphocytes using anti-Helios (clone 22F6) PE-CyTM7.")
![Flow Cytometry: Helios Antibody (22F6) [NBP2-37723] - Human peripheral blood lymphocytes were stained with anti-CD4 Pacific BlueTM, fixed and permeabilized with Treg Detection Kit and then intracellulary stained with anti-Helios PE (clone 22F6).](http://images.novusbio.com/fullsize/Helios-Antibody-22F6-Flow-Cytometry-NBP2-37723-img0002.jpg)
![SDS-Page: Recombinant Human Helios Protein [H00022807-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Helios-Protein-SDS-Page-H00022807-Q01-img0002.jpg "SDS-Page: Recombinant Human Helios Protein [H00022807-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Helios Antibody [NBP1-79703] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Helios-Antibody-Western-Blot-NBP1-79703-img0003.jpg "Western Blot: Helios Antibody [NBP1-79703] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: HELIOS Overexpression Lysate (Adult Normal) [NBL1-11892] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HELIOS.](http://images.novusbio.com/fullsize/HELIOS-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11892-img0002.jpg "Western Blot: HELIOS Overexpression Lysate (Adult Normal) [NBL1-11892] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HELIOS.")